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A colorimetric probe based on 4-mercaptophenol and thioglycolic acid-functionalized gold nanoparticles for determination of phytic acid and Fe(III) ions
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-30 , DOI: 10.1007/s00604-020-04478-2 Ömer Kaan Koç 1, 2 , Ayşem Üzer 2 , Reşat Apak 2, 3
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-30 , DOI: 10.1007/s00604-020-04478-2 Ömer Kaan Koç 1, 2 , Ayşem Üzer 2 , Reşat Apak 2, 3
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As a first of its kind, we developed a highly sensitive colorimetric nanoprobe for phytic acid (PA) and Fe(III) ion detection based on 4-mercaptophenol (4MP) and thioglycolic acid (TGA)-functionalized gold nanoparticles {AuNPs@(4MP-TGA)}. AuNPs were easily derivatized by 4MP and TGA through –SH binding to gold. Fe(III) ions possibly are bound first to the phenolate groups of 4MP-AuNPs, and further coordinated several nanoparticles via the carboxylate groups of TGA-AuNPs to cause aggregation, resulting in a red-to-purple color change and a bathochromic shift in the SPR absorption band of the nanoprobe. With the addition of PA to the AuNPs@(4MP-TGA)-Fe(III) system, the aggregated particles were released due to strong complex formation between Fe(III) and PA, resulting in a restoration of the color (purple-to-red) and of the SPR band to the original 520 nm wavelength maximum. Thus, the 650-nm absorption is attenuated and the 520-nm band is enhanced upon PA-Fe(III) chelation. This means that the absorption ratio A650/A520 is an indication of Fe(III) whereas the reverse ratio A520/A650 of the PA content of complex samples. The limits of detection (LOD) of the AuNPs@(4MP-TGA) were 1.0 μM for Fe(III) ions and 0.15 μM for PA. Phytic acid extracted from bean grains was determined with the proposed probe, yielding good recoveries. In addition, common metal ions, anions, and several biomolecules did not show an adverse effect on the nanoprobe performance for ferric ions and phytate. The developed method was statistically validated against a LC–MS/MS literature method. Graphical abstract Mercaptophenolate (4MP)- and thioglycolic acid (TGA)-functionalized gold nanoparticles were prepared as nanoprobes to detect Fe(III) ions through nanoparticle aggregation accompanied by red-to-purple color shift. The same nanoprobe determined phytic acid in food through disaggregation of Fe(III)-aggregated nanoparticles by strong Fe(III)-phytate chelation and restoration of solution color from purple to red. Mercaptophenolate (4MP)- and thioglycolic acid (TGA)-functionalized gold nanoparticles were prepared as nanoprobes to detect Fe(III) ions through nanoparticle aggregation accompanied by red-to-purple color shift. The same nanoprobe determined phytic acid in food through disaggregation of Fe(III)-aggregated nanoparticles by strong Fe(III)-phytate chelation and restoration of solution color from purple to red.
中文翻译：
基于 4-巯基苯酚和巯基乙酸官能化金纳米粒子的比色探针，用于测定植酸和 Fe(III) 离子
作为同类产品中的首创，我们开发了一种基于 4-巯基苯酚 (4MP) 和巯基乙醇酸 (TGA) 功能化金纳米粒子 {AuNPs@(4MP) 的高灵敏度比色纳米探针，用于植酸 (PA) 和 Fe(III) 离子检测-TGA)}。AuNPs 很容易被 4MP 和 TGA 通过-SH 与金的结合而衍生化。Fe(III) 离子可能首先与 4MP-AuNPs 的酚盐基团结合，并通过 TGA-AuNPs 的羧酸盐基团进一步配位几个纳米颗粒以引起聚集，导致红色到紫色的颜色变化和红移纳米探针的 SPR 吸收带。随着 PA 添加到 AuNPs@(4MP-TGA)-Fe(III) 体系中，由于 Fe(III) 和 PA 之间形成强烈的复合物，聚集的颗粒被释放，导致颜色（紫色到红色）和 SPR 波段恢复到原始 520 nm 波长最大值。因此，PA-Fe(III) 螯合后 650 nm 吸收减弱，520 nm 波段增强。这意味着吸收比 A650/A520 是 Fe(III) 的指示，而复杂样品的 PA 含量的反向比 A520/A650。AuNPs@(4MP-TGA) 的检测限 (LOD) 对于 Fe(III) 离子为 1.0 μM，对于 PA 为 0.15 μM。使用建议的探针测定了从豆粒中提取的植酸，获得了良好的回收率。此外，常见的金属离子、阴离子和几种生物分子对铁离子和植酸盐的纳米探针性能没有显示出不利影响。所开发的方法针对 LC-MS/MS 文献方法进行了统计验证。图形摘要 巯基苯酚 (4MP) 和巯基乙酸 (TGA) 功能化的金纳米粒子被制备为纳米探针，通过纳米粒子聚集检测 Fe(III) 离子，伴随着红色到紫色的色移。相同的纳米探针通过强 Fe(III)-植酸盐螯合作用分解 Fe(III)-聚集的纳米粒子，并将溶液颜色从紫色恢复为红色，从而测定食物中的植酸。将巯基苯酚 (4MP) 和巯基乙酸 (TGA) 功能化的金纳米颗粒制备为纳米探针，通过纳米颗粒聚集并伴随红到紫色的色移来检测 Fe(III) 离子。相同的纳米探针通过强 Fe(III)-植酸盐螯合作用分解 Fe(III)-聚集的纳米粒子，并将溶液颜色从紫色恢复为红色，从而测定食物中的植酸。
更新日期：2020-09-30
中文翻译：
基于 4-巯基苯酚和巯基乙酸官能化金纳米粒子的比色探针，用于测定植酸和 Fe(III) 离子
作为同类产品中的首创，我们开发了一种基于 4-巯基苯酚 (4MP) 和巯基乙醇酸 (TGA) 功能化金纳米粒子 {AuNPs@(4MP) 的高灵敏度比色纳米探针，用于植酸 (PA) 和 Fe(III) 离子检测-TGA)}。AuNPs 很容易被 4MP 和 TGA 通过-SH 与金的结合而衍生化。Fe(III) 离子可能首先与 4MP-AuNPs 的酚盐基团结合，并通过 TGA-AuNPs 的羧酸盐基团进一步配位几个纳米颗粒以引起聚集，导致红色到紫色的颜色变化和红移纳米探针的 SPR 吸收带。随着 PA 添加到 AuNPs@(4MP-TGA)-Fe(III) 体系中，由于 Fe(III) 和 PA 之间形成强烈的复合物，聚集的颗粒被释放，导致颜色（紫色到红色）和 SPR 波段恢复到原始 520 nm 波长最大值。因此，PA-Fe(III) 螯合后 650 nm 吸收减弱，520 nm 波段增强。这意味着吸收比 A650/A520 是 Fe(III) 的指示，而复杂样品的 PA 含量的反向比 A520/A650。AuNPs@(4MP-TGA) 的检测限 (LOD) 对于 Fe(III) 离子为 1.0 μM，对于 PA 为 0.15 μM。使用建议的探针测定了从豆粒中提取的植酸，获得了良好的回收率。此外，常见的金属离子、阴离子和几种生物分子对铁离子和植酸盐的纳米探针性能没有显示出不利影响。所开发的方法针对 LC-MS/MS 文献方法进行了统计验证。图形摘要 巯基苯酚 (4MP) 和巯基乙酸 (TGA) 功能化的金纳米粒子被制备为纳米探针，通过纳米粒子聚集检测 Fe(III) 离子，伴随着红色到紫色的色移。相同的纳米探针通过强 Fe(III)-植酸盐螯合作用分解 Fe(III)-聚集的纳米粒子，并将溶液颜色从紫色恢复为红色，从而测定食物中的植酸。将巯基苯酚 (4MP) 和巯基乙酸 (TGA) 功能化的金纳米颗粒制备为纳米探针，通过纳米颗粒聚集并伴随红到紫色的色移来检测 Fe(III) 离子。相同的纳米探针通过强 Fe(III)-植酸盐螯合作用分解 Fe(III)-聚集的纳米粒子，并将溶液颜色从紫色恢复为红色，从而测定食物中的植酸。
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