Indoles, including 5-methoxy-2-methyl-indole (5MeO2MeIn), are a potent trigger of strobilation in jellyfish polyps. Indoles may be an alternative method to cooling to induce strobilation, but the ephyrae produced should have similar or better rates of survival, deformities, growth, and behaviour to those produced by cooling, and polyps should remain viable after strobilating. We used two experiments to optimise the use of 5MeO2MeIn to induce strobilation in Aurelia coerulea (Scyphozoa). First, we compared rates of strobilation, survival, and deformities of ephyrae and budding of polyps exposed to 1.25, 2.5, and 5.0 µM 5MeO2MeIn for 3 days to cooling at 14 °C. Polyps in the indole and cooled treatments strobilated after 10 days and produced similar numbers of ephyrae, but more ephyrae were deformed in the 5.0 µM treatment and survival of ephyrae was lower in the 2.5 and 5.0 µM treatments than the cooled treatment. Polyps exposed to all three concentrations of indoles failed to bud or died after strobilation. Next, we exposed polyps to 0.7, 1.25, and 2.5 µM 5MeO2MeIn for four hours. Polyps exposed to all indole concentrations strobilated and produced similar numbers of ephyrae, but more ephyrae were deformed in the 2.5 µM treatment. Survival, behaviour, and sizes of ephyrae were similar 7 and 14 days after strobilation (although ephyrae pulsed faster in the 1.25 µM treatment at 14 days) and budding rates were similar in the indole and cooled treatments. Thus, exposing polyps to 0.7–1.25 µM 5MeO2MeIn for 4 h is a viable and efficient alternative to cooling to induce strobilation in polyps.

吲哚类化合物，包括5-甲氧基-2-甲基吲哚（5MeO2MeIn），是水母息肉中强力刺激的诱因。吲哚可能是冷却以诱导松毛的另一种方法，但是所产生的e菌的存活，畸形，生长和行为速率应与冷却所产生的相似或更好，并且息肉在松毛后应保持活力。我们使用了两个实验，以优化利用5MeO2MeIn的诱导strobilation奥里利亚犁头（裂殖体）。首先，我们比较了暴露于1.25、2.5和5.0 µM 5MeO2MeIn 3天并冷却至14°C的息肉的裂，存活率，and的变形率以及息肉的出芽率。吲哚和冷却处理后的息肉在10天后出现疲劳，并产生相似数量的e，但5.0 µM处理中更多的e变形，而2.5和5.0 µM处理中的survival的存活率低于冷却处理。暴露于所有三种浓度的吲哚的息肉在芽裂后不能发芽或死亡。接下来，我们将息肉暴露于0.7、1.25和2.5 µM 5MeO2MeIn 4小时。暴露于所有吲哚浓度的息肉会不稳定并产生相似数量的e，但在2.5 µM处理中会变形更多的e。生存，行为，窒息后7天和14天，days的大小和大小相似（尽管在14天时1.25 µM处理中，e的脉动更快），吲哚和冷处理的出芽率也相似。因此，将息肉暴露于0.7–1.25 µM 5MeO2MeIn 4 h是一种可行且有效的替代方法，可用来冷却息肉以引起息肉。