Waterborne diseases have critical public health issues and socioeconomic relevancy worldwide. Various viral pathogens are ordinarily associated with waterborne diseases. Six-year-surveillance (a total of 20 times) of norovirus, hepatitis A virus, group C rotavirus, and enterovirus was conducted at five raw water sampling sites including two lakes (Lakes Soyang and Juam), Hyundo region of Geum River in Daejeon City, and Guui region of Han River in Seoul Metropolitan City and Moolgeum region of Nakdong River in Gimhae City which are located near two water intake plants. In this study, we routinely investigated virus contamination in water samples through reverse transcription polymerase chain reaction (RT-PCR) and integrated cell culture RT-PCR with high sensitivity and specificity. A total 100 samples were tested. Most of the targeted viruses were found in 32% of the samples and at least one of the indicator bacteria was detected in 65% of these occurrences. Among all the detected viruses, enterovirus was the most prevalent with a detection frequency of 12% and 2.71 MPN/10 L on average, while hepatitis A virus was the least prevalent with a detection frequency of 4%. Nearly all of the analyzed viruses (except for group C rotavirus) were present in samples from Han River (the Guui region), Geum River (the Hyundo region), Lake Juam, and Nakdong River (the Moolgeum region), while group C rotavirus was detected in those from the Guui region. During the six-year sampling period, the targeted waterborne viruses in water samples exhibited seasonal patterns in their occurrence that were different from the indicator bacteria levels in the water samples. The fact that they were detected in the five representative Korean water environments makes it necessary to establish the chemical and biological analysis systems for waterborne viruses and sophisticated management systems.

水传播疾病在全球范围内都具有至关重要的公共卫生问题和社会经济意义。各种病毒病原体通常与水传播疾病有关。在大田锦江的两个湖（Soyang和Juam湖），五个水源采样点对诺如病毒，甲型肝炎病毒，C组轮状病毒和肠病毒进行了为期六年的监视（共20次）位于首尔市的汉江市和居贵地区以及位于金海市的那洞河的Moolgeum地区和金海市的这两个地区均位于两个取水厂附近。在这项研究中，我们常规地通过反转录聚合酶链反应（RT-PCR）和具有高灵敏度和特异性的集成细胞培养RT-PCR研究了水样中的病毒污染。总共测试了100个样品。在32％的样品中发现了大多数目标病毒，在这些事件的65％中至少检测到一种指示细菌。在所有检测到的病毒中，肠道病毒的流行率最高，平均检出率为12％，平均检出率为2.71 MPN / 10 L，而甲型肝炎病毒的流行率最低，检出率为4％。几乎所有分析的病毒（C组轮状病毒除外）都存在于汉江（Guui地区），锦江（Hyundo地区），Juam湖和Nakdong河（Moolgeum地区）的样本中，而C组轮状病毒在居伊地区的人中被发现。在六年的采样期内，目标水中的目标水传播病毒的出现具有季节性特征，与季节性指示细菌水平不同。