Terminators and introns are vital regulators of gene expression in many eukaryotes; however, the functional importance of these elements for controlling gene expression in Agaricomycetes remains unclear. In this study, the effects of Ceriporiopsis subvermispora terminators and introns on the expression of a recombinant hygromycin B phosphotransferase gene (hph) were characterized. Using a transient transformation system, we proved that a highly active terminator (e.g., the gpd terminator) is required for the efficient expression of the hph gene. Mutational analyses of the C. subvermispora gpd terminator revealed that hph expression was dictated by an A-rich region, which included a putative positioning element, and polyadenylation sites. In contrast, our results indicated that introns are not required for the expression of hph directed by the Csβ1-tub and Csgpd promoters in C. subvermispora. This study provides insights into the functions and cis-element requirements of transcriptional terminators in Agaricomycetes, which may be relevant for designing recombinant genes for this important fungal class.

终止子和内含子是许多真核生物中基因表达的重要调节剂。然而，这些元件对于控制姬松霉菌中基因表达的功能重要性尚不清楚。在这项研究中，表征了Ceriporiopsis subvermispora终止子和内含子对重组潮霉素B磷酸转移酶基因（hph）表达的影响。使用瞬时转化系统，我们证明了高效表达hph基因需要高活性的终止子（例如gpd终止子）。对C. subvermispora gpd终止子的突变分析表明，hph表达是由一个富含A的区域决定的，该区域包括一个假定的定位元件和聚腺苷酸化位点。相比之下，我们的结果表明，内含子不需要用于表达HPH通过定向Csβ1桶和Csgpd在启动子C. subvermispora。这项研究提供了洞察到的功能和顺式元件，要求在姬松露菌中转录终止子，这可能与设计此重要真菌类的重组基因有关。