Abstract—

Enzymes play significant roles in both life and industry, and increasing enzymatic activity has been a long-term goal of the biochemical science. Recently, it has been reported that nanomaterials can enhance both the specificity and yield of polymerase chain reaction (PCR) though the mechanism of this enhancement remained unclear. By using gold nanoparticles (AuNPs) as model nanomaterials and the cellulase as a typical enzyme, we studied whether and how nanomaterials affect enzymatic activities investigating various aspects of the reaction process. The results showed that the catalytic efficiency of cellulase depends on the concentration of AuNPs. By detailed studying the relation between catalysis and experimental conditions, we found that the enzymatic activity can be increased up to 13.13% upon adding AuNPs in concentration of 0.5 nM into the reaction solution. The optimal pH, temperature, and incubation time for cellulose with 0.5 nM AuNPs were found to be same as those for the cellulose alone. These findings might be useful for enhancing enzymatic activities in the industrial processes.

中文翻译：

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对提高催化效率的金纳米颗粒的全面理解

摘要-

酶在生命和工业中均起着重要作用，提高酶活性一直是生物化学的长期目标。近来，已经报道了纳米材料可以增强聚合酶链反应（PCR）的特异性和产率，尽管这种增强的机理仍不清楚。通过使用金纳米颗粒（AuNPs）作为模型纳米材料和纤维素酶作为典型酶，我们研究了纳米材料是否以及如何影响酶活性，从而研究了反应过程的各个方面。结果表明，纤维素酶的催化效率取决于AuNPs的浓度。通过详细研究催化与实验条件之间的关系，我们发现添加浓度为0的AuNPs时，酶活性可提高至13.13％。5 nM到反应溶液中。发现具有0.5 nM AuNP的纤维素的最佳pH，温度和孵育时间与单独的纤维素相同。这些发现对于增强工业过程中的酶活性可能是有用的。