Background:

The purpose of this study was to examine the effects of exposure to imatinib in the prenatal period on testis development in rats.

Methods:

Although all the study groups received intraperitoneal imatinib on prenatal days 1–8, no pregnancy occurred in the Imatinib-80 group. Immunohistochemical analysis, TUNEL, c-kit and PDGF staining revealed no difference between the groups in terms of positivity scoring.

Results:

A significant decrease was detected in total sperm counts in the Imatinib-20 group compared to the control group, but the sperm count was higher in the Imatinib-60 group than in the Imatinib-20 group. At biochemical measurements, the drug increased oxidative stress in the testis and serum in the Imatinib-20 group, but caused a decrease in tissue in the Imatinib-60 group. Thiol measurements revealed a decrease in the testis and serum in the Imatinib-60 group, while an increase in serum measurements was observed in the Imatinib-40 group. Analysis revealed no difference between the groups in terms of protamine and histone gene expression levels in testis tissue exposed to imatinib.

Conclusion:

Our findings show that prenatal exposure to imatinib can lead to histopathological and biochemical changes in testis tissue, but that no adverse effect occurs in nuclear maturation of germ cells during spermiogenesis.

中文翻译：

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产前给予伊马替尼对大鼠睾丸发育的影响

背景：

本研究的目的是检查产前暴露于伊马替尼对大鼠睾丸发育的影响。

方法：

尽管所有研究组均在产前第 1-8 天接受腹腔注射伊马替尼，但 Imatinib-80 组未发生妊娠。免疫组织化学分析、TUNEL、c-kit 和 PDGF 染色显示两组之间在阳性评分方面没有差异。

结果：

与对照组相比，Imatinib-20 组的总精子数量显着减少，但 Imatinib-60 组的精子数量高于 Imatinib-20 组。在生化测量中，该药物增加了 Imatinib-20 组睾丸和血清中的氧化应激，但导致 Imatinib-60 组组织减少。硫醇测量显示 Imatinib-60 组的睾丸和血清减少，而 Imatinib-40 组的血清测量增加。分析显示，暴露于伊马替尼的睾丸组织中的鱼精蛋白和组蛋白基因表达水平在各组之间没有差异。

结论：

我们的研究结果表明，产前暴露于伊马替尼可导致睾丸组织发生组织病理学和生化变化，但在精子发生过程中生殖细胞的核成熟不会发生不良影响。