Two experiments were performed for evaluating calibration curve (CC) and comparing negative and positive controls (CNP) as a major method for estimating of phytase phosphorus equivalence for layer and broiler chickens. In the first and second experiments, 360 Hy-line W-36 layer hens and 525 day-old Ross-308 broiler chickens were used in a complete randomized design, respectively. Evaluated methods were setting the two regression equations for NPP-supplemented and phytase supplemented treatments with two sub-methods, include calibration curve (CC) or exclude the amount of phosphorus content of the basal diet (CC-BD) in calculation, and exploring enzyme equivalency by comparing phosphorus deficient diet as a negative and supplemented diet by inorganic phosphorus sources as a positive control group (CNP). Experiment one included nine treatments (200, 300, 400, and 500 FTU/kg phytase was added to a phosphorus-deficient basal diet contained 0.12% AvP, the rest four treatments were included basal diet supplemented with 0.20, 0.27, 0.35, and 0.43% AvP). Experiment two included seven treatments (a basal P deficient diet contained 0.27% AvP, and two increasing levels of AvP, 0.32 and 0.37%, and four doses of phytase 200, 300, 400 and 500 FTU/kg added to basal diet). Each treatment in both experiments replicated five times. Results indicated that methods of estimation had a significant effect on phosphorus equivalence estimation (P<0.0001). Fitted regression equations considering P content of basal diet (CC-BD) estimated rational values than those ignore it (CC) (0.161% vs 0.365% and 0.432% vs 0.564% for 500 FTU/kg phytase for broiler chicken and layer hens, respectively) (P<0.0001). On average, among three methods used, the CC method had the highest estimated values both in broiler chickens and layer hens (p<0.0001). Regardless of the mathematical method, there were different significant values for different strains (layer, 0.381%, and broiler, 0.179%) (P<0.0001), but not for different traits served as response criteria (P>0.05). In conclusion, the phosphorus equivalent value of the enzyme varies according to the estimation methods and strain. Hence, using matrix values of the enzyme for accurate feed formulation depend on a variety of circumstances and decision making requires comprehensive information.

中文翻译：

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估算方法是否会影响蛋鸡和肉鸡植酸酶的磷当量值？

进行了两个实验，用于评估校准曲线（CC）和比较阴性对照和阳性对照（CNP），这是评估蛋鸡和肉鸡植酸酶磷当量的主要方法。在第一个和第二个实验中，分别在完全随机设计中使用了360只Hy-line W-36蛋鸡和525日龄的Ross-308肉鸡。评估的方法是设置NPP补充和植酸酶补充处理的两个回归方程，其中包括两个子方法，包括计算校准曲线（CC）或排除基础饮食中的磷含量（CC-BD），以及探索酶通过比较缺磷饮食作为阴性饮食和补充磷饮食与无机磷源作为阳性对照组（CNP）的等效性。实验一包括九种疗法（200种，将300、400和500 FTU / kg肌醇六磷酸酶添加到含0.12％AvP的缺磷基础饲料中，其余四种处理均包括补充0.20、0.27、0.35和0.43％AvP的基础饲料。实验二包括七种处理方法（基础P缺乏饮食含0.27％AvP，两种水平的AvP分别为0.32和0.37％，向基础饮食中添加了四剂量的植酸酶200、300、400和500 FTU / kg）。两个实验中的每种处理重复五次。结果表明，估算方法对磷当量估算有显着影响（P <0.0001）。考虑基础饮食中P含量的拟合回归方程（CC-BD）估计的合理值比忽略基础饮食（CC）的合理值（肉鸡500 FTU / kg植酸酶分别为0.161％比0.365％和0.432％比0.564％） ）（P <0.0001）。平均而言，在所使用的三种方法中，CC方法在肉鸡和蛋鸡中的估计值最高（p <0.0001）。不管采用哪种数学方法，不同菌株（蛋鸡，0.381％，肉鸡，0.179％）的显着性值均不同（P <0.0001），但不同性状作为响应标准（P> 0.05）。总之，酶的磷当量值根据估计方法和菌株而变化。因此，将酶的基质值用于精确的饲料配方取决于多种情况，而决策需要全面的信息。不同品系（蛋鸡，0.381％，肉鸡，0.179％）有不同的显着性值（P <0.0001），但不同性状作为响应标准（P> 0.05）。总之，酶的磷当量值根据估计方法和菌株而变化。因此，将酶的基质值用于精确的饲料配方取决于多种情况，而决策需要全面的信息。不同品系（蛋鸡，0.381％，肉鸡，0.179％）有不同的显着性值（P <0.0001），但不同性状作为响应标准（P> 0.05）。总之，酶的磷当量值根据估计方法和菌株而变化。因此，将酶的基质值用于精确的饲料配方取决于多种情况，而决策需要全面的信息。