Abstract

Aim

The objective of this study was to evaluate the probable mechanism of action of Biochanin A (BCA) on high-fat diet-induced insulin resistance mice.

Methods

Twenty-four male C57/BL/6J mice were divided into two and fed either control diet or a high fat-high diet (HFD). After 6 weeks, mice were grouped into: Control, Control + BCA, HFD and HFD + BCA (n = 6). Mice were made diabetic by feeding them an HFD.

Results

Body weight, plasma glucose, insulin, leptin levels and HOMA values were significantly lower in the BCA supplemented HFD group when related to the HFD group. Furthermore, BCA supplementation significantly increased Insulin receptor substrate 1 (IRS 1), PI3K, Akt and Glucose transporter type 4 (GLUT-4) protein abundance in skeletal muscle when equated with HFD.

Conclusion

This study demonstrates that BCA can improve insulin sensitivity by activating insulin signalling, suggesting that it may possess antidiabetic activities.

中文翻译：

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Biochanin A，一种大豆异黄酮，通过调节高脂肪饮食诱导的糖尿病小鼠的胰岛素信号通路来降低胰岛素抵抗

摘要

目的

本研究的目的是评估生物素 A (BCA) 对高脂肪饮食诱导的胰岛素抵抗小鼠的可能作用机制。

方法

将 24 只雄性 C57/BL/6J 小鼠分成两只，喂食对照饮食或高脂高饮食 (HFD)。6 周后，小鼠分为：对照、对照 + BCA、HFD 和 HFD + BCA（n  = 6）。通过喂食 HFD 使小鼠患上糖尿病。

结果

当与 HFD 组相关时，补充 BCA 的 HFD 组的体重、血浆葡萄糖、胰岛素、瘦素水平和 HOMA 值显着降低。此外，当等同于 HFD 时，补充 BCA 可显着增加骨骼肌中胰岛素受体底物 1 (IRS 1)、PI3K、Akt 和葡萄糖转运蛋白 4 (GLUT-4) 蛋白丰度。

结论

这项研究表明 BCA 可以通过激活胰岛素信号传导来提高胰岛素敏感性，表明它可能具有抗糖尿病活性。