Abstract

In the current scenario of overuse of pesticides (resulting in soil and water pollution and ultimately leading to biomagnification), a research project was carried out to study biodegradation of Triazophos. For this purpose, three bacterial strains (Pseudomonas kilonensis MB490, Pseudomonas kilonensis MB498 and Pseudomonas sp. MB504), isolated from cotton fields of Mianwali, Pakistan were investigated for Triazophos degradation and metabolite formation in M-9 broth, soil slurry and soil microcosm after incubation for 9 days. There was 88.4–95.8% Triazophos degradation in M-9 broth, 99.90% degradation in soil slurry and 92.74 to 96% Triazophos degradation in soil microcosm by these bacteria after 9 days. While there was negligible Triazophos degradation (upto 7%) in the controls without bacteria. According to GCMS analysis, 7 unique and novel metabolites (1, 2, 4-Triazole-4—amine, N-(2-Thienylmethyl), Benzene sulfonic acid hydrazide, Benzene sulfonic acid methyl ester, 4H-1,2,4-Triazole-4-benzenesulfonamide, 4, 5 dihydro-N-(O-toyl)-3-furamide, Ethyl 4-phenyldiazenylbenzoate and Dibutyl methanephosphonate) of Triazophos were revealed. Current results strongly suggest the potential of these bacterial strains for the remediation of Triazophos contaminated agricultural soils.

摘要

在目前农药过度使用（导致土壤和水污染并最终导致生物放大）的情况下，开展了一项研究项目，以研究三唑磷的生物降解。为此，使用了三种细菌菌株（千株假单胞菌MB490，千株假单胞菌MB498和假单胞菌sp。孵育9天后，从巴基斯坦Mianwali棉田分离出的MB504（MB504）被用于三唑磷降解和M-9肉汤，土壤浆液和土壤微观世界中代谢产物的形成。9天后，这些细菌在M-9肉汤中有38.4–95.8％的三唑磷降解，在土壤浆液中有99.90％的降解，在土壤微观世界中有92.74至96％的三唑磷降解。在没有细菌的对照中，三唑磷的降解可忽略不计（最高7％）。根据GCMS分析，有7种独特且新颖的代谢产物（1、2、4-三唑4-胺，N-（2-噻吩甲基），苯磺酸酰肼，苯磺酸甲酯，4H-1,2,4-揭示了三唑磷的三唑-4-苯磺酰胺，4，5二氢-N-（O-甲苯基）-3-呋喃酰胺，4-苯基二氮烯基苯甲酸乙酯和甲膦酸二丁酯。