Aedes mosquitoes can efficiently transmit many pathogenic arboviruses, placing a great burden on public health worldwide. In addition, they also carry a number of insect-specific viruses (ISVs), and it was recently suggested that some of these ISVs might form a stable species-specific “core virome” in mosquito populations. However, little is known about such a core virome in laboratory colonies and if it is present across different developmental stages. In this study, we compared the viromes in eggs, larvae, pupae, and adults of Aedes albopictus mosquitoes collected from a lab colony and compared each to the virome of different developmental stages collected in the field. The virome in lab-derived A. albopictus was very stable across all stages, consistent with a vertical transmission route of these viruses, and formed a possible “vertically transmitted core virome.” The different stages of field-collected A. albopictus mosquitoes also contained this stable vertically transmitted core virome, as well as another set of viruses (e.g., viruses distantly related to Guadeloupe mosquito virus, Hubei virga-like virus 2, and Sarawak virus) shared by mosquitoes across different stages, which might represent an “environment-derived core virome.” To further study this core set of ISVs, we screened 48 publicly available SRA viral metagenomic data sets of mosquitoes belonging to the genus Aedes, showing that some of the identified ISVs were identified in the majority of SRAs and providing further evidence supporting the core-virome concept.

中文翻译：

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实验室和现场收集的白纹伊蚊不同发育阶段病毒组的稳定性和伊蚊公开可用病毒组数据中可能的核心病毒

伊蚊可有效传播多种病原虫媒病毒，给全球公共卫生带来巨大负担。此外，它们还携带许多昆虫特异性病毒 (ISV)，最近有人提出，其中一些 ISV 可能在蚊子种群中形成稳定的物种特异性“核心病毒组”。然而，人们对实验室菌落中的这种核心病毒组以及它是否存在于不同的发育阶段知之甚少。在这项研究中，我们比较了从实验室菌落收集的白纹伊蚊的卵、幼虫、蛹和成虫中的病毒组，并将每种病毒组与野外收集的不同发育阶段的病毒组进行了比较。实验室衍生的A. albopictus 中的病毒组在各个阶段都非常稳定，与这些病毒的垂直传播途径一致，并形成了可能的“垂直传播的核心病毒组”。野外采集的白纹伊蚊不同阶段也含有这种稳定的垂直传播的核心病毒组，以及另一组病毒（例如，与瓜德罗普蚊病毒、湖北类病毒 2 和沙捞越病毒有远亲关系的病毒）。由不同阶段的蚊子组成，这可能代表了“环境衍生的核心病毒组”。为了进一步研究这组核心 ISV，我们筛选了属于伊蚊属的 48 个公开可用的 SRA 病毒宏基因组数据集，表明在大多数 SRA 中确定了一些已识别的 ISV，并提供了支持核心病毒组概念的进一步证据。