Transgelin (TAGLN) Regulates IQGAP1 and Alters the Functions of Breast Cancer Cells,Bulletin of the Korean Chemical Society

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Transgelin (TAGLN) Regulates IQGAP1 and Alters the Functions of Breast Cancer Cells
Bulletin of the Korean Chemical Society ( IF 2.3 ) Pub Date : 2020-09-29 , DOI: 10.1002/bkcs.12104
Bernardo R. Raymundo ₁ , In‐Rok Oh ₁ , Ling Xiu ₁ , Chan‐Wha Kim _{1,

2}

Affiliation

Several studies have been conducted on the transgelin (TAGLN) protein and its critical role in cancer biology. However, the regulation of this protein and the way in which this regulation is correlated with the functions of IQ motif‐containing GTPase‐activating protein 1 (IQGAP1) in MDA‐MB231 cells, remain unclear. We generated stable TAGLN‐knockdown and TAGLN‐overexpressing cells. These cells, along with their control counterparts, were cultured in the presence or absence of 17‐AAG. The different cell groups were then subjected to functional assays to assess proliferation, chemotaxis, and invasion. TAGLN regulation was found to affect the efficacy of 17‐AAG. The ability of TAGLN to influence the levels of IQGAP1 and its binding partners altered the critical functions of breast cancer cells. Therefore, the altered functionality of MDA‐MB‐231 cells, as a consequence of TAGLN regulation, is correlated with IQGAP1 signaling.

中文翻译：

Transgelin（TAGLN）调节IQGAP1并改变乳腺癌细胞的功能

已经对转蛋白（TAGLN）蛋白及其在癌症生物学中的关键作用进行了一些研究。然而，尚不清楚该蛋白的调节以及该调节与MDA-MB231细胞中含IQ基序的GTPase激活蛋白1（IQGAP1）的功能相关的方式。我们生成了稳定的TAGLN基因敲低和TAGLN基因过度表达的细胞。这些细胞及其对照对应物在有或没有17-AAG的条件下培养。然后，对不同的细胞组进行功能分析，以评估其增殖，趋化性和侵袭性。发现TAGLN调节会影响17-AAG的功效。TAGLN影响IQGAP1及其结合伴侣水平的能力改变了乳腺癌细胞的关键功能。因此，MDA-MB-231单元的功能发生了变化，

更新日期：2020-10-19

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