The objective of this research is to determine whether intramammary antibiotics with complementary acupuncture can reduce bovine mammary inflammation due to subclinical mastitis. Lactating cows were selected based on milk with a somatic cell count (SCC) greater than 500,000 cells/ml. Pre‐ and post‐treatment milk samples were collected to determine SCC, aerobic bacterial content, milk ion conductivity, total protein, lactate dehydrogenase (LDH) and N‐acetyl‐beta‐D‐glucosaminidase (NAGase) concentrations. Milk serum was prepared from milk samples by double centrifugation. Concentrations of LDH and NAGase were determined using commercial enzyme‐linked immunosorbent assays. Cows being treated with intramammary antibiotics were separated by random assignment to the acupuncture group (n = 10) and a no‐acupuncture (control) group (n = 9). Both the acupuncture and control group were restrained for 30 min in a head catch 12 hr apart for a total of four times. For front quarters affected by subclinical mastitis, the acupuncture points used were spleen (SP) 12, SP 17, SP 18, SP 21, stomach (ST) 18 and conception vessel (CV) 12. For rear quarters affected by subclinical mastitis, the acupuncture points used were bladder (BL) 30, BL 30‐1, BL 49, kidney (KI) 10, conception vessel (CV) 2 and CV 3. All parameters were compared using a Student t test. Significance was defined as p < .05. Compared to control cows, complementary acupuncture treatment reduced NAGase enzymatic activity in quarters of cows with subclinical mastitis. The reduction in NAGase suggests that complementary acupuncture treatment may be associated with healing of the damaged mammary epithelial cells, which are the primary source of NAGase activity in milk serum.

中文翻译：

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补充针灸的乳内抗生素可降低亚临床乳腺炎的奶牛的乳汁N-乙酰-β-D-氨基葡萄糖苷酶浓度

这项研究的目的是确定补充针灸的乳内抗生素是否可以减轻亚临床乳腺炎引起的牛乳腺炎症。泌乳牛的选择是基于体细胞计数（SCC）大于500,000细胞/ ml的牛奶。收集预处理前后的牛奶样品以确定SCC，需氧细菌含量，牛奶离子电导率，总蛋白，乳酸脱氢酶（LDH）和N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）浓度。通过两次离心从牛奶样品中制备牛奶血清。LDH和NAGase的浓度使用商业酶联免疫吸附测定法确定。通过随机分配针灸组将接受乳房内抗生素治疗的母牛分开（n = 10）和非针灸（对照组）组（n  = 9）。针刺组和对照组均在相距12小时的头部中进行30分钟的约束，共四次。对于受亚临床乳腺炎影响的前半部，使用的穴位为脾（SP）12，SP 17，SP 18，SP 21，胃（ST）18和受孕血管（CV）12。对于受亚临床乳腺炎影响的后半部，使用的穴位是膀胱（BL）30，BL 30-1，BL 49，肾脏（KI）10，受孕血管（CV）2和CV3。所有参数均使用Student t检验进行比较。重要性定义为p <.05。与对照牛相比，补充针灸治疗降低了亚临床性乳腺炎母牛中NAGase的酶活性。NAGase的减少表明补充针灸治疗可能与受损的乳腺上皮细胞的愈合有关，乳腺上皮细胞是牛奶血清中NAGase活性的主要来源。