Consecutive Silver(I) Ion Incorporation into Oligonucleotides containing Cytosine‐Cytosine Mispairs,ChemPlusChem

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Consecutive Silver(I) Ion Incorporation into Oligonucleotides containing Cytosine‐Cytosine Mispairs
ChemPlusChem ( IF 3.0 ) Pub Date : 2020-09-28 , DOI: 10.1002/cplu.202000607
M Nur Hossain ₁ , Syed Ahmad _{1,

2} , Heinz-Bernhard Kraatz _{1,

2}

Affiliation

Herein, the consecutive incorporation of Ag^I ion into the dsDNA containing adjacent C−C mispairs is demonstrated. The melting temperature (T_m) was 8 ^°C higher for DNA containing three C−C mispairs upon the addition of three Ag^I ions as compared to the Ag^I‐free DNA, and no T_m was obtained in the presence of excess Ag^I ion, indicating a stable bridging of C‐Ag^I−C upon the incorporation of the stoichiometric amount of Ag^I per C−C mispair. The circular dichroism (CD) spectra of the dsDNA showed a negative peak at ∼270 nm in the presence of excess Ag^I, implying that significant structural changes and a potential aggregation of DNA occurred. Subsequently, the Ag^I‐mediated DNA strands are immobilized on Au surfaces. Their electrochemical properties are monitored using CV, EIS and SECM showing increased overpotentials and charge‐transfer resistances, and decreased the rate constant in the presence of an excess of Ag^I, respectively. These results are further supported by the XPS and sulfide‐Au reductive desorption measurements.

中文翻译：

连续银 (I) 离子掺入含有胞嘧啶-胞嘧啶错配的寡核苷酸

在此，证明了将 Ag ^I离子连续掺入含有相邻 C-C 错配的 dsDNA 中。与不含Ag ^{I 的}DNA相比，添加三个 Ag ^I离子后含有三个 C−C 错配的 DNA的解链温度 (T _m )高8 ^° C ，并且在存在过量 Ag 的情况下没有获得T _m^I离子，表明在每个 C-C 错配加入化学计量量的 Ag ^I后，C-Ag ^I -C的稳定桥接。在过量 Ag ^I存在下，dsDNA 的圆二色性 (CD) 光谱在~270 nm 处显示负峰，这意味着发生了显着的结构变化和 DNA 的潜在聚集。随后，Ag ^I介导的 DNA 链被固定在 Au 表面。使用 CV、EIS 和 SECM 监测它们的电化学性质，分别显示出增加的过电位和电荷转移电阻，并在过量的 Ag ^I存在下降低了速率常数。XPS 和硫化物-金还原解吸测量进一步支持了这些结果。

更新日期：2020-09-28

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