Surgical resection of skin tumors leads to large defects in surrounding normal tissues, which should be reconstructed thereafter using the patient's own tissues taken from the other site. Our challenge is to solve this problem in dermal malignant melanoma (MM) by a novel process, named extracorporeal high pressure therapy (EHPT), in which the tissue containing tumor is resected and pressurized, and the treated tissue is re-transplant back to the same position as a tumor-free autologous dermal substitute. The key points are complete tumor death and preservation of native extra cellular matrix (ECM) by the hydrostatic pressure. We found that high hydrostatic pressure at 200 MPa for 10 min at room temperature is completely cytocidal against MM cells in suspension form, in monolayer form, and even in the solid tumor form. MM tumor-bearing nude mice were established by injected human MM cells intradermally and treated by EHTP. The denaturation of the dermal extra cellular matrices was so mild that the pressurized skin was well engrafted as tumor free autologous dermal tissues, resulting in the complete eradication of the MM without any unnecessary skin reconstruction surgery. This very simple and short pressing treatment was proved to make the tumor tissue to the transplantable and tumor-free autologous dermal substitute, which can be applicable to the other temporally resectable tissues.

皮肤肿瘤的手术切除会导致周围正常组织的大缺陷，此后应使用从其他部位获取的患者自己的组织进行重建。我们的挑战是通过一种名为体外高压疗法（EHPT）的新方法来解决真皮恶性黑色素瘤（MM）中的这一问题，在该方法中，将包含肿瘤的组织切除并加压，然后将经过治疗的组织重新移植回原位。与无肿瘤的自体真皮替代品的位置相同。关键点是完全的肿瘤死亡和静水压力对天然细胞外基质（ECM）的保存。我们发现，室温下200 MPa的高静水压持续10分钟对悬液形式，单层形式甚至实体瘤形式的MM细胞具有完全杀灭细胞的作用。通过皮内注射人MM细胞建立具有MM肿瘤的裸鼠，并用EHTP治疗。真皮细胞外基质的变性是如此温和，以至于加压皮肤被很好地植入为无肿瘤的自体真皮组织，从而完全根除了MM，而无需进行任何不必要的皮肤重建手术。事实证明，这种非常简单且短时间的压迫处理使肿瘤组织成为可移植且无肿瘤的自体真皮替代物，该替代物可应用于其他可在时间上切除的组织。从而彻底消除了MM，而无需进行任何不必要的皮肤重建手术。事实证明，这种非常简单且短时间的压迫处理使肿瘤组织成为可移植且无肿瘤的自体真皮替代物，该替代物可应用于其他可在时间上切除的组织。从而彻底消除了MM，而无需进行任何不必要的皮肤重建手术。事实证明，这种非常简单且短时间的压迫处理使肿瘤组织成为可移植且无肿瘤的自体真皮替代物，该替代物可应用于其他可在时间上切除的组织。