Abstract Lignification is an important inducement of quality deterioration of postharvest Flammulina velutipes. In this study, phenylpropanoid pathway and mitochondrial reactive oxygen species (ROS) metabolisms were taken as breakthrough points to investigate the lignification mechanism in harvested F. velutipes packaged in nanocomposite packaging material (Nano-PM). In comparison with polyethylene packaging material (Normal-PM) and no packaging material (No-PM), Nano-PM prevented the decrease of L* value and the increase of firmness. Nano-PM also maintained a better microstructure. Furthermore, Nano-PM reduced the deposition of lignin by suppressing the enzymes activities involved in phenylpropanoid pathway. In addition, the regulation of ROS in mitochondria alleviated the accumulation of Ca2+ and inhibited the programmed cell death (PCD) process, which ultimately delayed the lignification process. These results indicated that Nano-PM delayed the lignification process by regulating phenylpropanoid pathway and the accumulation of ROS in mitochondria.

中文翻译：

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纳米复合包装通过调节苯丙烷途径和线粒体活性氧代谢来延迟金针菇的木质化

摘要 木质化是金针菇采后品质下降的重要诱因。在这项研究中，苯丙烷途径和线粒体活性氧 (ROS) 代谢作为突破点，研究了包装在纳米复合包装材料 (Nano-PM) 中收获的 F. velutipes 的木质化机制。与聚乙烯包装材料（Normal-PM）和无包装材料（No-PM）相比，Nano-PM阻止了L*值的降低和坚固度的增加。Nano-PM 还保持了更好的微观结构。此外，Nano-PM 通过抑制苯丙烷途径中涉及的酶活性来减少木质素的沉积。此外，线粒体中ROS的调节减轻了Ca2+的积累并抑制了程序性细胞死亡（PCD）过程，最终延迟了木质化过程。这些结果表明，Nano-PM 通过调节苯丙烷途径和线粒体中 ROS 的积累来延迟木质化过程。