A marked prozone effect was observed in indirect immunofluorescence with human sera and human cerebrospinal fluid in two clinical cases involving breast carcinoma with paraneoplastic neuronal antibodies, and anti- N-methyl-D-aspartic acid (NMDA) receptor antibodies. Anti-Yo antibodies and anti-NMDA antibodies were not detectable under high concentrations (1:10 serum dilution and neat CSF respectively) but showed a true effect when sufficiently diluted at 1:80 and 1:5 respectively. This paper demonstrates that prozone effects have their occurrences in indirect immunofluorescence, and clinicians and laboratory technicians should be wary of its implications during screening of autoantibody markers in neurological diseases.

中文翻译：

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通过针对神经元抗原的抗体在间接免疫荧光测定中观察到前带现象

在涉及具有副肿瘤神经元抗体和抗 N-甲基-D-天冬氨酸 (NMDA) 受体抗体的乳腺癌的两个临床病例中，在人血清和人脑脊液的间接免疫荧光中观察到显着的前带效应。抗 Yo 抗体和抗 NMDA 抗体在高浓度（分别为 1:10 血清稀释度和纯 CSF）下检测不到，但在分别以 1:80 和 1:5 充分稀释时显示出真正的效果。本文证明前带效应在间接免疫荧光中发生，临床医生和实验室技术人员在筛选神经系统疾病自身抗体标志物时应注意其影响。