Salmonella enterica serovar Enteritidis (Salmonella Enteritidis [SE]) is a foodborne pathogen that is capable of affecting shell eggs and is regulated by Title 21 of the Code of Federal Regulations. A reservoir for this pathogen is the ceca of the gastrointestinal tract in chickens. In the present study, layer-type pullets in 2 consecutive studies were not treated or treated with a feed additive that comprised a synbiotic blend of probiotic (direct-fed microbial) and prebiotic ingredients at total dietary inclusion of 250 or 500 mg kg⁻¹ and were challenged orally with SE. At 10 d after challenge, pullets were euthanized and ceca were harvested for enumeration of SE. Pullets treated with Avi-Lution at 250 mg kg⁻¹ were found to have a significantly (P < 0.001) lower number of SE per g of cecal contents, where SE was approximately 1.0 log lower than control in experiment 1 and > 2.0 log lower than control in experiment 2. Pullets treated with Avi-Lution at 500 mg kg⁻¹ also had significantly lower burden of SE in cecal contents than control, and SE burden was not significantly different between Avi-Lution treatment at 250 and 500 mg kg⁻¹. The reduction of SE burden in the cecal reservoir demonstrated in the present study is expected to relate to decreased external contamination of eggs by SE in laying hens and therefore is presented as a food safety precaution where intervention in live birds against SE contamination is desired.

肠炎沙门氏菌肠炎沙门氏菌（Salmonella Enteritidis [ SE]）是一种食源性病原体，能够影响带壳蛋，并受《联邦法规》第21篇的规管。这种病原体的储存器是鸡胃肠道盲肠。在本研究中，连续2项研究中的蛋鸡没有经过饲料添加剂的处理或处理，该添加剂包含益生菌（直接饲喂微生物）和益生元的合生元混合物，日粮总摄入量为250或500 mg kg ^-1并与SE进行了口头挑战。攻击后第10天，对小母鸡实施安乐死，并收获盲肠进行SE计数。用Avi-Lution处理的小鸡在250 mg kg ^-1发现 每克盲肠内含物的SE数量显着降低（P <0.001），其中SE比实验1的对照低约1.0 log，比实验2的对照低2.0 log。在500 mg kg ^-1时，盲肠中的SE负担也显着低于对照，并且在250和500 mg kg ^-1的Avi-Lution处理之间，SE负担没有显着差异。本研究表明，减少盲肠水库中SE的负担与降低蛋鸡对SE的鸡蛋的外部污染有关，因此可作为食品安全预防措施，要求对活禽进行SE污染干预。