当前位置：
X-MOL 学术
›
Cryobiology
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Can amides be alternative cryoprotectors for the preservation of feline semen?
Cryobiology ( IF 2.3 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.cryobiol.2020.09.004 Mónica Madrigal-Valverde 1 , Rodrigo F Bittencourt 2 , Antônio de Lisboa Ribeiro Filho 2 , Gediendson R Araujo 3 , Maicon P Lents 2 , Elisiane S Santos 2 , Adrielle S Lima 2 , Paulo Mattos 4
Cryobiology ( IF 2.3 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.cryobiol.2020.09.004 Mónica Madrigal-Valverde 1 , Rodrigo F Bittencourt 2 , Antônio de Lisboa Ribeiro Filho 2 , Gediendson R Araujo 3 , Maicon P Lents 2 , Elisiane S Santos 2 , Adrielle S Lima 2 , Paulo Mattos 4
Affiliation
|
Sperm cryopreservation is a tool for the conservation of the genetic material of animals of genetic importance or for species preservation. In the case of domestic cats, this can be used to generate information about seminal harvest, evaluation and preservation, which is especially important due to its applicability to wild felids. This study evaluated seminal samples harvested by urethral catheterisation from 13 adult domestic cats. Samples were cryopreserved with experimental groups of extenders were defined by the penetrating cryoprotectant: 6% glycerol (GLY6%), 3% dimethylacetamide (DMA3%) and 3% dimethylformamide (DMF3%). The samples were thawed and evaluated by conventional microscopy and by computer-assisted sperm analysis (CASA). The structural and functional membrane integrity was assessed by supravital tests (EOS), hypoosmotic swelling tests (HOST) and flow cytometry (FC). There was a correlation (P < 0.05) between total motility and EOS (r = 0.54), HOST and FC (r = -0.62) and total motility and flow cytometry (r = 0.63), indicating that these are complementary parameters that increase the accuracy of the feline sperm quality evaluation post-thaw. The results regarding the structural and functional integrity of the sperm plasma membrane did not differ (P > 0.05) among groups. However, the DMA3% group had a lower (P < 0.05) percentage of morphological changes in the sperm tail compared to samples cryopreserved with GLY6% and DMF3%. Additionally, DMA3% provided lower values of immobile sperm post-thaw when compared to DMF3%. DMA is an interesting alternative to GLY and superior to DMF for the cryopreservation of feline semen at the studied concentrations.
中文翻译：
酰胺可以作为保存猫精液的替代冷冻保护剂吗？
精子冷冻保存是保存具有遗传重要性的动物的遗传物质或用于物种保存的工具。就家猫而言，这可用于生成有关精液收获、评估和保存的信息，由于其适用于野生猫科动物，这一点尤其重要。这项研究评估了通过导尿管从 13 只成年家猫身上采集的精液样本。样品被冷冻保存，实验组的扩展剂由渗透性冷冻保护剂定义：6% 甘油 (GLY6%)、3% 二甲基乙酰胺 (DMA3%) 和 3% 二甲基甲酰胺 (DMF3%)。将样品解冻并通过常规显微镜和计算机辅助精子分析 (CASA) 进行评估。通过超生命测试（EOS）评估结构和功能膜的完整性，低渗肿胀试验（HOST）和流式细胞术（FC）。总运动性和 EOS (r = 0.54)、HOST 和 FC (r = -0.62) 以及总运动性和流式细胞术 (r = 0.63) 之间存在相关性 (P < 0.05)，表明这些是增加解冻后猫精子质量评估的准确性。关于精子质膜结构和功能完整性的结果在各组之间没有差异（P > 0.05）。然而，与用 GLY6% 和 DMF3% 冷冻保存的样品相比，DMA3% 组精子尾部的形态变化百分比较低 (P < 0.05)。此外，与 DMF3% 相比，DMA3% 在解冻后提供了较低的不动精子值。
更新日期：2020-12-01
中文翻译：
酰胺可以作为保存猫精液的替代冷冻保护剂吗？
精子冷冻保存是保存具有遗传重要性的动物的遗传物质或用于物种保存的工具。就家猫而言，这可用于生成有关精液收获、评估和保存的信息，由于其适用于野生猫科动物，这一点尤其重要。这项研究评估了通过导尿管从 13 只成年家猫身上采集的精液样本。样品被冷冻保存，实验组的扩展剂由渗透性冷冻保护剂定义：6% 甘油 (GLY6%)、3% 二甲基乙酰胺 (DMA3%) 和 3% 二甲基甲酰胺 (DMF3%)。将样品解冻并通过常规显微镜和计算机辅助精子分析 (CASA) 进行评估。通过超生命测试（EOS）评估结构和功能膜的完整性，低渗肿胀试验（HOST）和流式细胞术（FC）。总运动性和 EOS (r = 0.54)、HOST 和 FC (r = -0.62) 以及总运动性和流式细胞术 (r = 0.63) 之间存在相关性 (P < 0.05)，表明这些是增加解冻后猫精子质量评估的准确性。关于精子质膜结构和功能完整性的结果在各组之间没有差异（P > 0.05）。然而，与用 GLY6% 和 DMF3% 冷冻保存的样品相比，DMA3% 组精子尾部的形态变化百分比较低 (P < 0.05)。此外，与 DMF3% 相比，DMA3% 在解冻后提供了较低的不动精子值。
京公网安备 11010802027423号