Growing interest in nanoparticles' (NPs) behavior in the environment needs deeper purport of interactions between NPs and amino acids. In this work, we evaluated particle size distribution, aggregation degree, zeta potential, adsorption efficiency, and specific adsorption of three ZnO NPs with average sizes 30, 135, and 210 nm in 1 M glycine solution at different pH. The interaction of ZnO NPs and glycine is stimulated by the pH growth from 4 to 8 (an increase of glycine adsorption and decrease of particle aggregation), whereas at рН > 8 colloidal and adsorption properties are weakly affected by pH change. The addition of glycine to water promotes the aggregation of 30 nm NPs, while the aggregation of bigger NPs is weakened. For smaller particles aggregation and adsorption efficiency are higher, and specific adsorption is lower, meanwhile, the size effect is stronger in aggressive medium: alkaline – in water, and acid – in glycine.

人们对环境中纳米颗粒（NPs）行为的兴趣日益浓厚，需要更深层次地关注NPs与氨基酸之间的相互作用。在这项工作中，我们评估了1M甘氨酸溶液在不同pH值下三种平均大小分别为30、135和210 nm的ZnO NP的粒径分布，聚集度，ζ电位，吸附效率和比吸附。pH值从4增长到8会刺激ZnO NP与甘氨酸的相互作用（甘氨酸吸附增加，颗粒聚集减少），而当pH> 8时，胶体和吸附特性受pH变化的影响很小。向水中添加甘氨酸可促进30 nm NP的聚集，而减弱较大NP的聚集。对于较小的颗粒，聚集和吸附效率较高，比吸附较低，同时，