A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin. This ECL-RET platform is composed of aptamer decorated BODIPY composites and C₆₀@BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with K₂S₂O₈. BODIPY derivative and K₂S₂O₈ are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process. At the same time, C₆₀, as the accelerator of K₂S₂O₈, can effectively increase the ECL signal and further transfer as much energy as possible to BODIPY derivative. Under optimal conditions, the constructed ECL-RET platform exhibited sensitive detection of lactoferrin in the wide linear range of 10^-4- 850 ng/mL with a LOD of 42 fg/mL. Meanwhile, the proposed ECL-RET aptasensor demonstrated superior stability, specificity and reproducibility, displaying favorable application value in practical diagnosis of this method.

提出了一种基于新型染料BODIPY衍生物的比例电化学发光共振能量转移（ECL-RET）平台，用于乳铁蛋白的快速检测。该ECL-RET平台由适配体修饰的BODIPY复合材料和C ₆₀ @BSA组成，其中BODIPY衍生物是ECL探针，可以与K ₂ S ₂ O ₈产生显着的共振能量转移。BODIPY导数和K ₂ S ₂ O ₈分别用作内置参考信号和校准信号，以通过双信号自校准过程消除背景信号和异常变化信号。同时C ₆₀作为K ₂的加速剂S ₂ O ₈可以有效地增加ECL信号，并进一步将尽可能多的能量传递给BODIPY导数。在最佳条件下，构建的ECL-RET平台表现出乳铁蛋白的灵敏的检测在10的线性范围宽^-4 - 850毫微克/毫升与42 FG / mL的LOD。同时，所提出的ECL-RET适体传感器表现出优异的稳定性，特异性和可重复性，在该方法的实际诊断中具有良好的应用价值。