The present study reports the synthesis of silver nanoparticles (AgNPs) using haloalkaliphilic Streptomyces spp. characterization, and antifungal activity thereof. The UV visible spectra of synthesized AgNPs showed a characteristic absorption peak at 430 nm, due to the excitation of Surface Plasmon Resonance. Scanning electron microscopy and transmission electron microscopy images showed spherical shape NPs with an average particle size of 16.4 ± 2.2 nm. The crystalline structure of the AgNPs was confirmed by X-ray diffraction (XRD). Zeta potential analyses revealed that NPs were negatively charged (− 8.12 ± 3.87 mV). The synthesized AgNPs are significantly active against phytopathogenic fungi, Fusarium verticillioides and Ustilago maydis. Microscopic, histo- and bio-chemical investigation of AgNPs against F. verticillioides revealed that AgNPs at 100 μg concentration inhibits the hyphal growth and conidia germination, and ~ 42.85% reduction of ergosterol biosynthesis. The results of propidium iodide staining and high relative cell membrane conductivity confirmed AgNPs mediated damage to the membrane. Moreover, the AgNPs synthesized by Streptomyces spp. inhibit the growth of F. verticillioides could be due to the inhibition of ergosterol biosynthesis and membrane damage. In our knowledge, this is the first report demonstrating the anti F. verticillioides activity of AgNPs synthesized by Streptomyces spp.

本研究报道了使用卤代嗜碱链霉菌属的spp合成银纳米颗粒（AgNPs）。表征及其抗真菌活性。由于表面等离振子共振的激发，合成的AgNPs的紫外可见光谱在430 nm处显示出特征吸收峰。扫描电子显微镜和透射电子显微镜图像显示球形NP，平均粒径为16.4±2.2 nm。AgNP的晶体结构通过X射线衍射（XRD）确认。Zeta电位分析显示NP带有负电荷（-8.12±3.87 mV）。合成的AgNP对植物病原真菌，尖镰刀菌和Ustilago maydis具有显着活性。微观的，A组织和针对的AgNPs的生物化学调查F.轮枝揭示的AgNPs以100μg浓度抑制菌丝生长和孢子萌发，和麦角固醇生物合成的〜42.85％的减少。碘化丙啶染色和较高的相对细胞膜电导率的结果证实了AgNPs介导的膜损伤。此外，由链霉菌属物种合成的AgNP 。抑制黄萎病菌的生长可能是由于抑制了麦角固醇的生物合成和膜破坏。在我们所知，这是第一份报告，证明反F.轮枝由合成的AgNPs的活性链霉菌属。