Gene expression profiles during the transition from fasting to refeeding were investigated in the gut and liver of Masu salmon Oncorhynchus masou masou. Fish were starved for 3 days and then fed at 3% of body weight. The gut and liver were resected before and after feeding, and the tissues were used for transcriptome analyses. Twenty-nine and 45 genes were more than eightfold differently expressed in the gut and liver, respectively. Genes involved in fatty acid and carbohydrate metabolism were differentially expressed in the liver tissues. Genes involved in protein folding, such as GRP78 and endoplasmin, were significantly upregulated in both organs at 6, 12, and 24 h after feeding. Furthermore, glycogen synthase kinase (GSK) 3-binding protein, a negative regulator of GSK with pivotal roles in stress response, was downregulated in both organs 6 h after feeding. These results suggest that the change in nutritional status from fasting to refeeding is accompanied by cellular stress.

中文翻译：

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饥饿-再喂养导致马苏鲑鱼的肠道和肝脏细胞应激反应 Oncorhynchus masou masou

在马苏鲑鱼 Oncorhynchus masou masou 的肠道和肝脏中研究了从禁食到再喂养过渡期间的基因表达谱。将鱼饥饿三天，然后以体重的 3% 喂食。在喂养前后切除肠道和肝脏，并将组织用于转录组分析。29 个和 45 个基因在肠道和肝脏中的表达差异分别超过 8 倍。参与脂肪酸和碳水化合物代谢的基因在肝组织中差异表达。在喂食后 6、12 和 24 小时，参与蛋白质折叠的基因，如 GRP78 和内质蛋白，在两个器官中均显着上调。此外，糖原合酶激酶 (GSK) 3 结合蛋白是 GSK 的负调节因子，在应激反应中起关键作用，进食后 6 小时在两个器官中均下调。这些结果表明，从禁食到再进食的营养状态变化伴随着细胞压力。