The immunolocalization of apoptotic factors in rat neocortex was studied on the model of permanent occlusion of the middle cerebral artery with administration of exogenous BDNF. We revealed heterogeneous distribution of pro- and anti-apoptotic factors in the stroke area and in the surrounding penumbra, where caspase-3+ and p53+ cells were found. Their number was maximum on day 3 of ischemia. The number of neurons containing anti-apoptotic factors was relatively decreased. Injection of BDNF changed the distribution of apoptotic factors. In the penumbra area, BDNF enhanced the expression of Mdm2 primarily in the pyramid cells of layers V/VI and Bcl-2 in interneurons of layers II and III. Localization of p53 and caspase-3 varied at different terms of the ischemic period and showed an inverse dependence. Considering the selective neuroprotective effect of BDNF, various mechanisms of the formation of ischemic tolerance in neurons are proposed.

中文翻译：

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脑源性神经营养因子 (BDNF) 作为局灶性实验性中风条件下细胞凋亡的调节剂

在外源性BDNF给药的大脑中动脉永久性闭塞模型上研究了大鼠新皮质中凋亡因子的免疫定位。我们揭示了中风区域和周围半暗带中促凋亡和抗凋亡因子的异质分布，其中发现了 caspase-3+ 和 p53+ 细胞。它们的数量在缺血的第 3 天达到最大值。含有抗凋亡因子的神经元数量相对减少。BDNF的注射改变了凋亡因子的分布。在半影区，BDNF 增强 Mdm2 的表达，主要是在 V/VI 层的锥体细胞和 II 和 III 层的中间神经元中的 Bcl-2 和 Bcl-2。p53 和 caspase-3 的定位在缺血期的不同时期有所不同，并显示出负相关性。