Bacteria mineralization is a promising biotechnological approach to apply in biomaterials development. In this investigation, we demonstrate that Bacillus subtilis 168 induces and influences CaCO₃ composites precipitation. Crystals were formed in calcium-carbon non-coupled (glycerol + CaCl₂, GLY; or glucose + CaCl₂, GLC) and coupled (calcium lactate, LAC; or calcium acetate, ACE) agar-sources, only maintaining the same Ca²⁺ concentration. The mineralized colonies showed variations in morphology, size, and crystallinity form properties. The crystals presented spherulitic growth in all conditions, and botryoidal shapes in GLC one. Birefringence and diffraction patterns confirmed that all biogenic carbonate crystals (BCC) were organized as calcite. The CaCO₃ in BCC was organized as calcite, amorphous calcium carbon (ACC) and organic matter (OM) of biofilm; all of them with relative abundance related to bacteria growth condition. BCC-GLY presented greatest OM composition, while BCC-ACE highest CaCO₃ content. Nucleation mechanism and OM content impacted in BCC crystallinity.

细菌矿化是一种有前途的生物技术方法，可用于生物材料的开发。在这项调查中，我们证明了枯草芽孢杆菌168诱导和影响CaCO ₃复合物的沉淀。晶体在非耦合的钙碳碳（甘油+ CaCl ₂，GLY；或葡萄糖+ CaCl ₂，GLC）和耦合的（乳酸钙，LAC；或醋酸钙，ACE）琼脂中形成，仅保持相同的Ca ^{2。 +}浓度。矿化的菌落显示出形态，大小和结晶形式特性的变化。晶体在所有条件下均呈球状生长，在GLC中呈晶体状。双折射和衍射图谱证实所有生物碳酸盐晶体（BCC）都组织为方解石。BCC中的CaCO ₃组织为生物膜中的方解石，无定形钙碳（ACC）和有机物（OM）。它们都与细菌生长状况有关。BCC-GLY表现出最大的OM组成，而BCC-ACE表现出最高的CaCO ₃含量。成核机理和OM含量影响BCC的结晶度。