Recent advances in microfluidic technologies for circulating tumor cells: enrichment, single-cell analysis, and liquid biopsy for clinical applications,Lab on a Chip

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Recent advances in microfluidic technologies for circulating tumor cells: enrichment, single-cell analysis, and liquid biopsy for clinical applications
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-09-28 , DOI: 10.1039/d0lc00577k
Haimeng Pei ₁ , Lu Li , Zhaojun Han , Yiguo Wang , Bo Tang

Affiliation

Circulating tumor cells (CTCs) detach from primary or metastatic lesions and circulate in the peripheral blood, which is considered to be the cause of distant metastases. CTC analysis in the form of liquid biopsy, enumeration and molecular analysis provide significant clinical information for cancer diagnosis, prognosis and therapeutic strategies. Despite the great clinical value, CTC analysis has not yet entered routine clinical practice due to lack of efficient technologies to perform CTC isolation and single-cell analysis. Taking the rarity and inherent heterogeneity of CTCs into account, reliable methods for CTC isolation and detection are in urgent demand for obtaining valuable information on cancer metastasis and progression from CTCs. Microfluidic technology, featuring microfabricated structures, can precisely control fluids and cells at the micrometer scale, thus making itself a particularly suitable method for rare CTC manipulation. Besides the enrichment function, microfluidic chips can also realize the analysis function by integrating multiple detection technologies. In this review, we have summarized the recent progress in CTC isolation and detection using microfluidic technologies, with special attention to emerging direct enrichment and enumeration in vivo. Further, few insights into single CTC molecular analysis are also demonstrated. We have provided a review of potential clinical applications of CTCs, ranging from early screening and diagnosis, tumor progression and prognosis, treatment and resistance monitoring, to therapeutic evaluation. Through this review, we conclude that the clinical utility of CTCs will be expanded as the isolation and analysis techniques are constantly improving.

中文翻译：

用于循环肿瘤细胞的微流技术的最新进展：富集，单细胞分析和临床活检

循环肿瘤细胞（CTC）与原发性或转移性病变分离并在外周血中循环，这被认为是远处转移的原因。液体活检，计数和分子分析形式的CTC分析为癌症诊断，预后和治疗策略提供了重要的临床信息。尽管具有巨大的临床价值，但由于缺乏进行CTC分离和单细胞分析的有效技术，CTC分析尚未进入常规临床实践。考虑到四氯化碳的稀有性和固有的异质性，迫切需要可靠的四氯化碳分离和检测方法，以从四氯化碳中获得有关癌转移和进展的有价值的信息。微流体技术，具有微细结构，可以精确地控制微米级的液体和细胞，因此使其本身特别适合用于罕见的CTC操作。除了富集功能外，微流控芯片还可以通过集成多种检测技术来实现分析功能。在这篇综述中，我们总结了使用微流体技术在四氯化碳分离和检测方面的最新进展，特别关注了新兴的直接富集和枚举体内。此外，还没有显示出对单一CTC分子分析的见解。我们对CTC的潜在临床应用进行了综述，范围从早期筛选和诊断，肿瘤进展和预后，治疗和耐药性监测到治疗评估。通过这次审查，我们得出结论，随着分离和分析技术的不断改进，四氯化碳的临床用途将得到扩展。

更新日期：2020-10-14

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