Miniature Multiwavelength Deep UV-LED-Based Absorption Detection System for Capillary LC,Analytical Chemistry

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Miniature Multiwavelength Deep UV-LED-Based Absorption Detection System for Capillary LC
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-09-28 , DOI: 10.1021/acs.analchem.0c03460
Mohamed Hemida _{1,

2} , Lewellwyn Joseph Coates _{1,

2,

3} , Shing Lam _{1,

2} , Vipul Gupta _{2,

4} , Mirek Macka ₂ , Hans-Jürgen Wirth ₃ , Andrew A. Gooley ₃ , Paul R. Haddad _{1,

2} , Brett Paull _{1,

2,

4}

Affiliation

A new miniature deep UV absorbance detector has been developed using low-cost and high-performance LEDs, which can be operated in both scanning (230 to 300 nm) and individual wavelength (240, 255, and 275 nm) detection modes. The detector is mostly composed of off-the-shelf components, such as LEDs, trifurcated fiber optic assembly, a capillary Z-type flow cell, and photodiodes. It has been characterized for use with a standard capillary LC system and was benchmarked against a standard variable wavelength capillary LC detector. The detector shows very low levels of stray light (<0.4%), utilization of up to 99.0% of the effective path length of the flow cell, a wide dynamic range (0.5 to 200 μg/mL for sulfamethazine, carbamazepine, and flavone), and low noise levels (at 300 μAU level). The detector was applied within a miniaturized LC system.

中文翻译：

基于微型多波长深紫外-LED的毛细管液相色谱吸收检测系统

使用低成本和高性能的LED开发了一种新型的微型深紫外吸收检测器，该检测器可以在扫描（230至300 nm）和单个波长（240、255和275 nm）两种检测模式下运行。检测器主要由现成的组件组成，例如LED，三叉光纤组件，毛细管Z型流通池和光电二极管。它的特点是可与标准毛细管LC系统一起使用，并已针对标准可变波长毛细管LC检测器进行了基准测试。检测器显示出极低的杂散光（<0.4％），流动池有效光程长度的高达99.0％的利用率，宽动态范围（磺胺二甲嘧啶，卡马西平和黄酮为0.5至200μg/ mL）和低噪声水平（在300μAU级别）。该检测器应用于小型LC系统中。

更新日期：2020-10-21

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