Assessing cell proliferation dynamics is crucial to understand the spatiotemporal control of organogenesis. Here we have generated a versatile fluorescent sensor, PlaCCI (plant cell cycle indicator) on the basis of the expression of CDT1a-CFP, H3.1-mCherry and CYCB1;1-YFP, that identifies cell cycle phases in Arabidopsis thaliana. This tool works in a variety of organs, and all markers and the antibiotic resistance are expressed from a single cassette, facilitating the selection in mutant backgrounds. We also show the robustness of PlaCCI line in live-imaging experiments to follow and quantify cell cycle phase progression.

中文翻译：

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用于拟南芥时空细胞周期分析的综合荧光传感器

评估细胞增殖动力学对于了解器官发生的时空控制至关重要。在这里，我们根据 CDT1a-CFP、H3.1-mCherry 和 CYCB1;1-YFP 的表达生成了一种多功能荧光传感器 PlaCCI（植物细胞周期指示器），可识别拟南芥中的细胞周期阶段。该工具适用于多种器官，所有标记物和抗生素抗性均由单个盒式磁带表达，便于在突变体背景中进行选择。我们还展示了 PlaCCI 线在实时成像实验中的稳健性，以跟踪和量化细胞周期阶段的进展。