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A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis
Nature Plants ( IF 15.8 ) Pub Date : 2020-09-28 , DOI: 10.1038/s41477-020-00770-4
Bénédicte Desvoyes 1 , Ainhoa Arana-Echarri 1 , María D Barea 1 , Crisanto Gutierrez 1
Affiliation  

Assessing cell proliferation dynamics is crucial to understand the spatiotemporal control of organogenesis. Here we have generated a versatile fluorescent sensor, PlaCCI (plant cell cycle indicator) on the basis of the expression of CDT1a-CFP, H3.1-mCherry and CYCB1;1-YFP, that identifies cell cycle phases in Arabidopsis thaliana. This tool works in a variety of organs, and all markers and the antibiotic resistance are expressed from a single cassette, facilitating the selection in mutant backgrounds. We also show the robustness of PlaCCI line in live-imaging experiments to follow and quantify cell cycle phase progression.



中文翻译:

用于拟南芥时空细胞周期分析的综合荧光传感器

评估细胞增殖动力学对于了解器官发生的时空控制至关重要。在这里,我们根据 CDT1a-CFP、H3.1-mCherry 和 CYCB1;1-YFP 的表达生成了一种多功能荧光传感器 PlaCCI(植物细胞周期指示器),可识别拟南芥中的细胞周期阶段。该工具适用于多种器官,所有标记物和抗生素抗性均由单个盒式磁带表达,便于在突变体背景中进行选择。我们还展示了 PlaCCI 线在实时成像实验中的稳健性,以跟踪和量化细胞周期阶段的进展。

更新日期:2020-09-28
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