We engineered Escherichia coli to grow on CO₂ and formic acid alone by introducing the synthetic CO₂ and formic acid assimilation pathway, expressing two formate dehydrogenase genes, fine-tuning metabolic fluxes and optimizing the levels of cytochrome bo₃ and bd-I ubiquinol oxidase. Our engineered strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO₂ and formic acid alone.

中文翻译：

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大肠杆菌被设计成在 CO 2 和甲酸上生长

我们通过引入合成的 CO ₂和甲酸同化途径，表达两种甲酸脱氢酶基因，微调代谢通量并优化细胞色素bo ₃和bd -I 泛醇氧化酶的水平，使大肠杆菌仅在 CO ₂和甲酸上生长. 我们的工程菌株可以在 450 小时内生长到 600 nm 处的光密度为 7.38，并显示出作为单独在 CO ₂和甲酸上生长的平台菌株的前景。