Abstract

This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical body condition score, which was then screened by a panel of diagnostic tests viz. Johnin, ELISA, fecal microscopy, and fecal culture, for the establishment of a case–control resource population. A total of four SNPs viz. rs208222804, rs211654540, rs208814257, and rs210748127 in CD209 gene were genotyped by PCR-RFLP. All SNPs, except rs210748127, were polymorphic in our population. Genotypic–phenotypic associations were assessed by the PROCLOGISTIC procedure of SAS 9.3. The SNP rs208814257 yielded three genotypes viz. CC, CG, and GG, which were significantly (p < 0.05) different in case as compared to the control population. The odds of CC and CG in comparison to GG genotype were 1.21 and 0.40, respectively. The CG genotype was significantly higher in control population, indicating that this genotype may provide resistance against PTB in our resource population. Upon validation in an independent, larger test population and following biological characterization, SNP rs208814257 can be incorporated in marker panel for selection of animals with greater resistance to MAP infection.

摘要

本研究旨在探讨CD209基因单核苷酸多态性(SNPs)与印度牛鸟分枝杆菌副结核亚种( MAP )引起的牛副结核病(PTB)的相关性。根据身体状况评分初步选择了213只动物，然后通过一组诊断测试进行筛选，即。Johnin、ELISA、粪便显微镜检查和粪便培养，用于建立病例对照资源群体。总共四个 SNP，即。CD209 中的 rs208222804、rs211654540、rs208814257 和rs210748127基因通过 PCR-RFLP 进行基因分型。除 rs210748127 外，所有 SNP 在我们的人群中都是多态的。通过 SAS 9.3 的 PROCLOGISTIC 程序评估基因型-表型关联。SNP rs208814257 产生了三种基因型，即。CC、CG 和 GG， 与对照组相比，病例显着 ( p < 0.05) 不同。与 GG 基因型相比，CC 和 CG 的几率分别为 1.21 和 0.40。对照人群中的 CG 基因型显着更高，表明该基因型可能在我们的资源人群中提供对 PTB 的抗性。在独立的、更大的测试群体中验证并遵循生物学特征后，可以将 SNP rs208814257 纳入标记组中，以选择具有更大抗性的动物MAP感染。