Abstract

A new alkaloid, manniindole 1, together with four known compounds: aristolactam AII 2, aristolactam BII 3, piperolactam D 4 and polycarpol 5 were isolated from the crude extract EtOH-H₂O (8:2) of the roots of Anonidium mannii by chromatographic separation. The structure elucidation was performed on the basis of a spectroscopic analysis (IR, HRESI MS, 1D and 2D NMR) as well as a comparison of their spectral data with those reported in the literature. For the first time, the crude extract and those isolated compounds were evaluated for their anti-schistosomal activity against Schistosoma mansoni and for cytotoxicity activity against Huh7 and A549 cells. Furthermore, they were also tested in vitro on the recent characterized Schistosoma mansoni NAD⁺ catabolizing enzyme (SmNACE) for their impact on this enzyme which is localized on the outer surface of the adult parasite. Compound 2 displayed quite good worm killing capability, while 4 showed significant inhibition of SmNACE.

摘要

一种新的生物碱，manniindole 1，连同四个已知化合物：aristolactam AII 2，aristolactam BII 3，piperolactam d 4和polycarpol 5是从粗提取物的EtOH-H隔离₂的根部：O（2 8）Anonidium mannii由色谱分离。结构解析是在光谱分析（IR、HRESI MS、1D 和 2D NMR）的基础上进行的，并将它们的光谱数据与文献中报道的数据进行比较。首次评估了粗提物和那些分离的化合物对曼氏血吸虫的抗血吸虫活性以及对 Huh7 和 A549 细胞的细胞毒性活性。此外，他们还在体外对最近表征的曼氏血吸虫NAD ⁺分解代谢酶 ( Sm NACE)进行了体外测试，以确定它们对这种位于成虫外表面的酶的影响。化合物2显示出相当好的杀虫能力，而化合物4显示出对Sm NACE的显着抑制。