ABSTRACT This study aimed to analyze the most important apple vitamin, L-ascorbic acid (AsA), and the most abundant fat-soluble one, α-tocopherol, in the pulp of 24 cultivars from harvest season 2010 and in six cultivars collected over three consecutive years (2008–2010). All cultivars were grown and stored under identical conditions and identified as ‘true to type’ by molecular genetic tools. The two vitamins were analyzed using high performance liquid chromatography with diode array detection (HPLC-DAD), involving two rapid methods with excellent recovery, linearity, precision, and sensitivity. Results showed an intriguing diversity of AsA contents and, to a lesser extent, of α-tocopherol in biological replicates, between cultivars and harvest years. Nevertheless, cultivar-specific relative vitamin contents can be deduced from this study. Among the 24 cultivars, a minimum AsA content of 0.43 ± 0.13 mg/100 g fresh weight (FW) and a maximum of 6.22 ± 1.06 mg/100 g FW have been found in ‘Gala’ and ‘Freiherr von Berlepsch,’ respectively. α-tocopherol ranged from 0.13 ± 0.02 mg/100 g FW to 0.33 ± 0.04 mg/100 g FW for ‘Freiherr von Berlepsch’ and ‘Brixner Plattling,’ respectively. The results of the present study suggest a potential of some old cultivars as a component of a healthy, vitamin-rich diet.

中文翻译：

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苹果果肉中的 L-抗坏血酸和α-生育酚含量：3 个收获季节 24 个品种和年度变化的比较

摘要 本研究旨在分析 2010 年收获季节的 24 个品种的果肉和三个以上收集的六个品种的果肉中最重要的苹果维生素 L-抗坏血酸 (AsA) 和最丰富的脂溶性维生素 α-生育酚。连续多年（2008-2010 年）。所有栽培品种均在相同条件下生长和储存，并通过分子遗传工具鉴定为“真实类型”。使用带二极管阵列检测器的高效液相色谱法 (HPLC-DAD) 分析这两种维生素，涉及两种具有出色回收率、线性、精密度和灵敏度的快速方法。结果显示，在栽培品种和收获年份之间，生物复制品中 AsA 含量和在较小程度上的 α-生育酚含量具有有趣的多样性。尽管如此，可以从这项研究中推断出特定品种的相对维生素含量。在这 24 个品种中，分别在“Gala”和“Freiherr von Berlepsch”中发现最低 AsA 含量为 0.43 ± 0.13 mg/100 g 鲜重 (FW)，最高为 6.22 ± 1.06 mg/100 g FW。对于“Freiherr von Berlepsch”和“Brixner Plattling”，α-生育酚的范围分别为 0.13 ± 0.02 mg/100 g FW 到 0.33 ± 0.04 mg/100 g FW。本研究的结果表明，一些古老的栽培品种有可能成为健康、富含维生素的饮食的组成部分。