ABSTRACT

We investigated possible changes in morphology and immunohistochemistry of the uterus and ovaries of rats caused by nandrolone (ND); we also investigated effects on fertility. We used 30 rats divided into three experimental groups: control (C), control vehicle (CV) and 5 mg/kg ND. Rats treated with ND exhibited loss of estrous cyclicity with predominance of the estrus phase, increased body weight and an organosomatic index that was decreased for the ovaries, but increased for the uterus. In the ovary, we observed a reduction in primary and secondary follicles and an increase in tertiary follicles; no corpora lutea were observed. Estrogen and progesterone levels were reduced. In the uterus, the endometrium was edematous with hyperplasic glands. The cytokines, TNFα and IL6, and the apoptotic index were increased in rats treated with ND. VEGF-A was increased in the ovaries and decreased in the uterus. We conclude that ND disrupts ovarian and uterine histophysiology by establishing an anovulatory and inflammatory condition, which directly affects reproduction.

摘要

我们研究了诺龙 (ND) 引起的大鼠子宫和卵巢的形态学和免疫组织化学的可能变化；我们还研究了对生育力的影响。我们使用了 30 只大鼠，分为三个实验组：对照 (C)、对照载体 (CV) 和 5 mg/kg ND。用 ND 治疗的大鼠表现出发情周期的丧失，主要是发情期，体重增加，卵巢器官指数下降，但子宫增加。在卵巢中，我们观察到初级和次级卵泡减少，三级卵泡增加；没有观察到黄体。雌激素和孕激素水平降低。在子宫内，子宫内膜水肿，腺体增生。用 ND 治疗的大鼠的细胞因子、TNFα 和 IL6 以及凋亡指数增加。VEGF-A 在卵巢中增加，在子宫中减少。我们得出结论，ND 通过建立直接影响生殖的无排卵和炎症状况来破坏卵巢和子宫的组织生理学。