Identification and analysis of micro‐exons in AP2/ERF and MADS gene families,FEBS Open Bio

当前位置： X-MOL 学术 › FEBS Open Bio › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Identification and analysis of micro‐exons in AP2/ERF and MADS gene families
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-09-28 , DOI: 10.1002/2211-5463.12990
Qi Song _{1,

2} , Amna Bari _{1,

2} , Huan Li _{1,

2} , Ling-Ling Chen _{1,

2}

Affiliation

Micro‐exons are a set of ultrashort exons with lengths ≤ 51 nucleotides. Our previous study revealed that micro‐exons were enriched in AP2 domains and K‐box domains, which are crucial components of AP2/ERF (APETALA2/ethylene‐responsive element‐binding protein) and MADS‐box (an acronym of MCM1, AGAMOUS, DEFICIENS and SRF) genes, respectively. In this study, we analyzed micro‐exons in the AP2/ERF family from 63 species and demonstrated that 76.8% of micro‐exons are concentrated in AP2 domains. Most micro‐exons appeared in the AP2 subfamily of all the terrestrial plants, but not algae. In addition, micro‐exons and AP2 domains are conserved and under negative selection. The MIKC gene is a typical MADS‐box gene family in terrestrial plants and includes one MADS‐box domain and one K‐box domain. A total of 92.3% of micro‐exons were observed in K‐box domains, and two micro‐exons usually encoded a region of K‐box domain, which is the key to MADS‐box protein polymerization. Furthermore, the micro‐exons of the K‐box domain had higher ratios of nonsynonymous mutations than those of the AP2 domains. Overall, here we explored the relationships and differences among micro‐exons in AP2/ERF and MADS families, and revealed potential functional roles of micro‐exons in these domains.

中文翻译：

AP2/ERF和MADS基因家族微外显子的鉴定与分析

微外显子是一组长度≤ 51 个核苷酸的超短外显子。我们之前的研究表明，微外显子富含 AP2 结构域和 K-box 结构域，它们是AP2 / ERF（APETALA2/乙烯反应元件结合蛋白）和MADS - box（MCM1、AGAMOUS、 DEFICIENS 和 SRF) 基因。在这项研究中，我们分析了来自 63 个物种的AP2 / ERF家族中的微外显子，并证明 76.8% 的微外显子集中在 AP2 域中。大多数微外显子出现在所有陆生植物的AP2亚科中，但不是藻类。此外，微外显子和 AP2 结构域是保守的并且处于负选择之下。这MIKC基因是陆生植物中典型的MADS -box基因家族，包括一个MADS-box结构域和一个K-box结构域。在 K-box 域中观察到总共 92.3% 的微外显子，两个微外显子通常编码 K-box 域的一个区域，这是 MADS-box 蛋白聚合的关键。此外，K-box 域的微外显子比 AP2 域的非同义突变率更高。总的来说，在这里我们探讨了AP2 / ERF和MADS家族中微外显子之间的关系和差异，并揭示了微外显子在这些领域的潜在功能作用。

更新日期：2020-12-03

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11