Off‐Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR,Advanced Materials Interfaces

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Off‐Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2020-09-28 , DOI: 10.1002/admi.202001074
Bing Shi _{1,

2} , Di Wu _{1,

2} , Yangyang Jiang ₁ , Junru An _{1,

2} , Wenming Wu _{1,

2}

Affiliation

High efficient and stable droplets preparation is an essential technology in digital PCR (dPCR) and other analytical fields. In this paper, a microfluidic device called vertical step emulsification (VSE) device to produce emulsion droplets rapidly off‐chip is developed. VSE device prepares droplets by perfluoroalkoxy (PFA) capillary and microcentrifuge tube, when the continuous water phase in PFA capillary jets into the static oil phase in the microcentrifuge tube at a certain speed, the continuous water phase is emulsified into stable droplets due to the sudden change of surface tension. In order to control the droplet size accurately, a general screw mechanism to control the gap between the PFA capillary outlet and the bottom of the inner wall of microcentrifuge tube is used. Meanwhile, dPCR tests with high dynamic range throughput droplets from VSE device are performed. The results show that the off‐chip droplet preparation device developed by the authors not only gets rid of the complicated control structure, has the advantages of low cost, easy operation, and user‐friendly, but also provides a sustainable development scheme for the preparation of high‐throughput and stable droplets in a short time.

中文翻译：

用于微滴数字PCR的片外垂直阶梯乳化微滴制备装置

高效稳定的液滴制备是数字PCR（dPCR）和其他分析领域的一项必不可少的技术。本文开发了一种称为垂直步进乳化（VSE）装置的微流体装置，该装置可快速在芯片外产生乳液液滴。VSE设备通过全氟烷氧基（PFA）毛细管和微量离心管制备液滴，当PFA毛细管中的连续水相以一定速度喷射到微量离心管中的静态油相中时，由于突然发生，连续水相被乳化成稳定的液滴表面张力的变化。为了精确地控制液滴的大小，使用了一般的螺旋机构来控制PFA毛细管出口和微量离心管内壁底部之间的间隙。与此同时，使用来自VSE设备的高动态范围通量液滴进行dPCR测试。结果表明，作者开发的片外液滴制备装置不仅摆脱了复杂的控制结构，具有成本低，操作简便，用户友好的优点，而且为制备提供了可持续的发展方案。在短时间内获得高通量和稳定的液滴。

更新日期：2020-11-21

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