Catalytically Active Copper Phosphate–Dextran Sulfate Microparticle Coatings for Bioanalyte Sensing,Particle & Particle Systems Characterization

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Catalytically Active Copper Phosphate–Dextran Sulfate Microparticle Coatings for Bioanalyte Sensing
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-09-28 , DOI: 10.1002/ppsc.202000210
Pietro Pacchin Tomanin ₁ , Sukhvir Kaur Bhangu _{1,

2} , Frank Caruso ₁ , Francesca Cavalieri _{2,

3}

Affiliation

Engineering reactive and functional nanostructured surfaces is important for enhancing the sensitivity and versatility of biosensors and microreactors. For example, the assembly of hybrid inorganic–organic porous microparticles on surfaces may provide a catalytic microenvironment for a wide range of reactions. Herein, the synthesis of catalytically active porous dextran sulfate–copper phosphate hybrid microparticles by a facile and rapid crystallization process in aqueous solution is reported. The sulfated polysaccharide enables control over the size and hierarchical morphology of the hybrid microparticles, as well as their assembly into stable macroporous coatings. The engineered microparticle coatings display intrinsic nonenzymatic peroxidase‐like catalytic activity when employed as a platform for the detection of hydrogen peroxide. Pairing of the microparticle coating with glucose oxidase affords a hybrid platform that is employed as a glucose sensor for monitoring physiological concentrations of a given analyte via a hybrid enzymatic/nonenzymatic cascade reaction. This work presents a strategy for the assembly of hybrid porous microparticles into enzyme‐mimicking surfaces for copper‐based catalysis and biochemical analyte sensing.

中文翻译：

用于生物分析物传感的催化活性磷酸铜-硫酸葡聚糖微粒涂层

工程反应性和功能性纳米结构表面对于提高生物传感器和微反应器的灵敏度和多功能性很重要。例如，混合无机-有机多孔微粒在表面的组装可以为广泛的反应提供催化微环境。本文报道了在水溶液中通过简便快速的结晶过程合成具有催化活性的多孔硫酸葡聚糖-磷酸铜杂化微粒。硫酸化多糖能够控制混合微粒的大小和分级形态，以及它们组装成稳定的大孔涂层。当用作检测过氧化氢的平台时，经过改造的微粒涂层显示出内在的非酶促过氧化物酶样催化活性。微粒涂层与葡萄糖氧化酶的配对提供了一个混合平台，该平台用作葡萄糖传感器，用于通过混合酶促/非酶促级联反应监测给定分析物的生理浓度。这项工作提出了一种将混合多孔微粒组装成用于铜基催化和生化分析物传感的酶模拟表面的策略。

更新日期：2020-09-28

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