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Artemia salina: A microcrustacean to assess engineered nanoparticles toxicity
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-09-28 , DOI: 10.1002/jemt.23609 Roberta Pecoraro 1 , Elena Maria Scalisi 1 , Giuseppina Messina 1 , Giuseppe Fragalà 1 , Sara Ignoto 1 , Antonio Salvaggio 2 , Massimo Zimbone 3 , Giuliana Impellizzeri 3 , Maria Violetta Brundo 1
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-09-28 , DOI: 10.1002/jemt.23609 Roberta Pecoraro 1 , Elena Maria Scalisi 1 , Giuseppina Messina 1 , Giuseppe Fragalà 1 , Sara Ignoto 1 , Antonio Salvaggio 2 , Massimo Zimbone 3 , Giuliana Impellizzeri 3 , Maria Violetta Brundo 1
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The focus of this work was to investigate the toxicity of different metal nanoparticles (gold nanoparticles [AuNPs], silver nanoparticles [AgNPs], titanium dioxide nanoparticles [TiO2NPs]) on brine shrimp Artemia salina. We investigated if nanoparticles could have an influence on hatching of cysts and on mortality of larvae. Larvae (also called nauplii) and cysts were exposed to NPs for 24 hr in artificial seawater on microplates. At the end of the test, we assessed the endpoint (immobility/death) for the larvae by a stereomicroscope. Nauplii that appeared completely motionless, were counted as dead, and the percentages of mortality were calculated for each treatment. Instead for the cysts, the percentages of not‐hatched nauplii for each concentration considered were calculated by counting the number of whole cysts. Currently, nanoparticles toxicity has been investigated in several research; in our study we highlighted the nontoxicity of TiO2NPs on A. salina nauplii as shown by low percentages of immobilization and on cysts because TiO2NPs do not affect their hatching. Despite AuNPs exerted toxic effects on hatching, they did not affect larvae development as well as TiO2NPs. Otherwise, AgNPs induced mortality of the larvae and inhibited cysts hatching.
中文翻译：
卤虫：一种用于评估工程纳米粒子毒性的微甲壳动物
这项工作的重点是研究不同金属纳米粒子（金纳米粒子 [AuNPs]、银纳米粒子 [AgNPs]、二氧化钛纳米粒子 [TiO 2 NPs]）对卤虫卤虫的毒性. 我们研究了纳米颗粒是否会对包囊的孵化和幼虫的死亡率产生影响。幼虫（也称为无节幼体）和包囊在微孔板上的人造海水中暴露于纳米颗粒 24 小时。在测试结束时，我们通过立体显微镜评估了幼虫的终点（不动/死亡）。看起来完全静止的无节幼体被计为死亡，并计算每种处理的死亡率百分比。对于包囊，通过计算整个包囊的数量来计算所考虑的每个浓度的未孵化无节幼体的百分比。目前，纳米粒子的毒性已经在多项研究中进行了研究；在我们的研究中，我们强调了 TiO 2 NPs 对A. salina的无毒由于 TiO 2 NPs 不影响它们的孵化，因此无节幼体的固定百分比低和在孢囊上。尽管 AuNPs 对孵化产生毒性作用，但它们并没有像 TiO 2 NPs一样影响幼虫的发育。否则，AgNPs 会导致幼虫死亡并抑制包囊孵化。
更新日期：2020-09-28
中文翻译：
卤虫：一种用于评估工程纳米粒子毒性的微甲壳动物
这项工作的重点是研究不同金属纳米粒子（金纳米粒子 [AuNPs]、银纳米粒子 [AgNPs]、二氧化钛纳米粒子 [TiO 2 NPs]）对卤虫卤虫的毒性. 我们研究了纳米颗粒是否会对包囊的孵化和幼虫的死亡率产生影响。幼虫（也称为无节幼体）和包囊在微孔板上的人造海水中暴露于纳米颗粒 24 小时。在测试结束时，我们通过立体显微镜评估了幼虫的终点（不动/死亡）。看起来完全静止的无节幼体被计为死亡，并计算每种处理的死亡率百分比。对于包囊，通过计算整个包囊的数量来计算所考虑的每个浓度的未孵化无节幼体的百分比。目前，纳米粒子的毒性已经在多项研究中进行了研究；在我们的研究中，我们强调了 TiO 2 NPs 对A. salina的无毒由于 TiO 2 NPs 不影响它们的孵化，因此无节幼体的固定百分比低和在孢囊上。尽管 AuNPs 对孵化产生毒性作用，但它们并没有像 TiO 2 NPs一样影响幼虫的发育。否则，AgNPs 会导致幼虫死亡并抑制包囊孵化。
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