Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence‐based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc‐Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc‐Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I_exp, mean maximum bioluminescence intensity of the experimental measurement (I_exp); I_c, luminescence intensity in control; I_exp/I_c, %) values for participants in the wrestler group while a decrease in the student group (P < 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.

中文翻译：

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一种无创且定性的生物发光测定法，用于快速诊断运动员对体力活动的反应

即将到来的职业体育主管部门正在寻求快速的无创生物传感工具，以定期监测运动员的生理状态。通过基于发光的生物传感器对唾液进行分析被认为是实现此目的的合适选择。本研究报告基于由细菌荧光素酶（BLuc）和烟酰胺腺嘌呤二核苷酸（NADH）：黄素单核苷酸（FMN）氧化还原酶（Red），BLuc‐Red组成的偶联酶系统的定性生物发光测定法，用于运动员的明确诊断劳累前后的压力水平。参加研究的志愿者分为自由式摔跤运动员和适应不同水平体育活动的学生。在体育锻炼模型条件下，研究了参与者唾液对BLuc‐Red催化的光发射的影响。结果显示残留发光量显着增加（I_exp，实验测量值的平均最大生物发光强度（I _exp）；I _c，对照中的发光强度；摔跤运动员组的参与者的I _exp / I _c，％）值，而学生组的参与者则有所下降（P <0.05）。发现两组中这种相反的残余发光值取决于唾液的过氧化氢酶活性。所提出的生物发光测定法可用作潜在的非特异性生物传感工具，用于确定高负荷下运动员的身体状态。