Abstract Simultaneous saccharification and fermentation (SSF) is a widely used approach for lignocellulosic butanol production. However, the major drawbacks for SSF lie in poor cellulase activity, sugar yield and butanol productivity at temperature below 37 °C. For the first time, a genetically engineered Clostridium acetobutylicum with enhanced thermotolerance at 39–45 °C was applied to high temperature SSF of corn stover pretreated by dilute sulfuric acid and aqueous ammonia. The optimized SSF at 42 °C with 12 h pre-hydrolysis gave 10.8 g/L butanol and 18.2 g/L ABE using pretreated solids and overlimed acid-treated liquid, obtaining butanol yield and productivity of 0.18 g/g-substrate and 0.18 g/L/h. Significant improvements on butanol production and productivity were achieved compared to conventional SSF process. The demonstrated SSF made full use of the sugars from acid pretreatment and acid-treated liquid for hydrolysis, reduced total cellulase usage, and contributed to hydrolysis and fermentation efficiency, indicating its potential for improving lignocellulosic butanol production.

中文翻译：

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玉米秸秆高温同步糖化发酵，通过耐热丙酮丁醇梭菌高效生产丁醇

摘要 同时糖化发酵 (SSF) 是一种广泛使用的木质纤维素丁醇生产方法。然而，SSF 的主要缺点在于在低于 37 °C 的温度下纤维素酶活性、糖产量和丁醇生产率较差。首次将在 39-45°C 下具有增强耐热性的基因工程丙酮丁醇梭菌应用于用稀硫酸和氨水预处理的玉米秸秆的高温 SSF。在 42 °C 下进行 12 小时预水解的优化 SSF 使用预处理的固体和过度酸处理的液体得到 10.8 g/L 丁醇和 18.2 g/L ABE，获得了 0.18 g/g-底物和 0.18 g 的丁醇产率和生产率/升/小时。与传统的 SSF 工艺相比，丁醇生产和生产率得到了显着改善。