Citrus canker is a major disease caused by Xanthomonas citri pv. citri. Snakin-1 is an antimicrobial peptide, which was previously shown to be effective against different bacterial and fungal diseases in potato, wheat and lettuce when expressed in transgenic plants. We generated transgenic Citrange Troyer citrus rootstocks constitutively expressing this peptide and 5 different transgenic lines were challenged against virulent X. citri isolates. Challenge assays conducted in vitro using detached leaves and in planta by infiltration revealed a significant reduction of the number and size of canker lesions in some of the transgenic lines.

柑橘溃疡病是由柑橘黄单胞菌引起的主要疾病。柑橘。Snakin-1 是一种抗菌肽，先前已证明当在转基因植物中表达时，它可以有效对抗马铃薯、小麦和生菜中的不同细菌和真菌病害。我们生成了组成型表达该肽的转基因 Citrange Troyer 柑橘砧木，并对 5 个不同的转基因品系进行了针对毒性柑橘属植物分离株的挑战。进行挑战的测定在体外使用离体叶片和在植物中通过渗透揭示了一些转基因品系的显著减少的数量和溃疡损伤的尺寸。