The genus Aphidius is one of the most commercially important species in greenhouse for controlling pests, especially aphids. Although 13 Aphidius species had been recorded in South Korea, it was not carefully surveyed yet. In this study, we surveyed and collected 8 Aphidius spp. including two unrecorded species. Using the DNA barcode region of mitochondrial cytochrome c oxidase subunit I (COI), sequences were newly generated for 16 molecular operational taxonomic units (MOTUs) of 8 species, except for 15 duplicates, in addition of 58 MOTUs of 18 species referred from GenBank for comparison and identification, which were reconstructed in a barcode phylogeny with Neighbor-Joining method. Then, to define each species, both Automatic Barcode Gap Discovery (ABGD) and Bayesian Poisson Tree Processes (bPTP) analyses were performed. As results, although most of Aphidius spp. could be identified by clustering into a clade, we recognized that three Aphdius species were placed in a clade, of which each species was not separated as an independent species. Our data suggested that those species demonstrating a species complex were able to be hypothesized as one species. In addition, diagnoses and illustrations of the two newly recorded species in South Korea were provided.

属烟蚜茧是在温室中最重要的商业物种防治害虫之一，特别是蚜虫。尽管韩国已记录了13种蚜虫，但尚未对其进行仔细调查。在这项研究中，我们调查并收集了8种蚜虫。包括两个未记录的物种。使用线粒体细胞色素C氧化酶亚基I（COI），重新生成了8种物种的16种分子操作生物分类单位（MOTU）的序列，除了15个重复样本外，还从GenBank转介了18种物种的58种MOTU，用于比较和鉴定，这些序列通过Neighbor-加盟方法。然后，为定义每个种类，执行了自动条形码间隔发现（ABGD）和贝叶斯泊松树过程（bPTP）分析。作为结果，虽然蚜虫属大多数。可以通过聚集成进化枝来识别，我们认识到三个Aphdius物种被放置在进化枝中，其中每个物种都没有作为一个独立的物种被分离。我们的数据表明，那些表现出物种复杂性的物种能够被假设为一个物种。此外，还提供了对韩国两个新记录物种的诊断和插图。