Hyperlipidemia-induced retinal vascular dysfunction is a complex pathological process. circRNAs are important regulators of biological processes and disease progression. However, the expression pattern of circRNAs in hyperlipidemia-induced retinal vascular dysfunction remains unclear. Herein, we used a murine model of hyperlipidemia and identified 317 differentially expressed circRNAs between hyperlipidemic retinas and normolipidemic retinas by circRNA microarrays. GO analysis indicated that the host genes of dysregulated circRNAs were targeted to cell differentiation (ontology: biological process), cytoplasm (ontology: cellular component), and protein binding (ontology: molecular function). Pathway analysis revealed that circRNAs-mediated network was mostly enriched in focal adhesion signaling. Notably, circLDB1 was significantly up-regulated in the serum of coronary artery disease patients and aqueous humor of age-related macular degeneration patients. circLDB1 regulated endothelial cell viability, proliferation, and apoptosis in vitro. Thus, circRNAs are the promising targets for the prediction and diagnosis of hyperlipidemia-induced vascular diseases.

高脂血症引起的视网膜血管功能障碍是一个复杂的病理过程。circRNA是生物过程和疾病进展的重要调节剂。然而，circRNA在高脂血症诱导的视网膜血管功能障碍中的表达模式仍不清楚。在此，我们使用了高脂血症的小鼠模型，并通过 circRNA 微阵列鉴定了 317 个在高脂血症视网膜和正常脂血症视网膜之间差异表达的 circRNA。GO分析表明，失调的circRNA的宿主基因靶向细胞分化（本体：生物过程）、细胞质（本体：细胞成分）和蛋白质结合（本体：分子功能）。通路分析表明，circRNAs 介导的网络主要富含粘着斑信号。尤其，circLDB1 在冠状动脉疾病患者的血清和老年性黄斑变性患者的房水中显着上调。circLDB1 在体外调节内皮细胞活力、增殖和凋亡。因此，circRNAs是预测和诊断高脂血症引起的血管疾病的有希望的靶点。