Chasteberry is highly recommended as an herbal medicine across the globe for treating of many gynaecological disorders. In this study, chasteberry extract (CBE) was supplemented in goldfish diet to evaluate the immunity responses at the cellular and molecular levels. Moreover, after the feeding trial, the fish were challenged with Aeromonas hydrophila. The fish (300 individuals, 2.4 ± 0.12 g initial weight) were randomly distributed in 12 tanks and were fed with 0 (control), 5, 10, and 15 g CBE per kg of feed for 8 weeks. Based on the results, lysozyme activity, alkaline phosphatase, and total immunoglobulin (Ig) in the skin mucus samples were significantly enhanced in the fish fed with 15 g/kg CBE (P < 0.05). Moreover, dietary CBE positively affected lysozyme activity, complement components, and IgM content of the serum samples compared to the control group. Also, the number of monocytes and lymphocytes were increased significantly with increasing CBE in the diet (P < 0.05). The highest mRNA levels of tumor necrosis factors (TNF-α, TNF-2α) and Lysozyme were observed in 15 g/kg CBE treatment. After the challenge test, the highest relative percentage survival value (60%) was observed in the fish fed with 15 g/kg CBE. We concluded that dietary CBE especially at 15 g/kg has an immunomodulatory effect in goldfish by stimulating the innate immunity and some inflammatory cytokines as well as disease resistance against A. hydrophila.

强烈建议在全球范围内将Chasteberry用作治疗许多妇科疾病的草药。在这项研究中，在金鱼饲料中添加了桔梗提取物（CBE）以评估细胞和分子水平的免疫应答。此外，在饲喂试验之后，将鱼用嗜水气单胞菌攻击。将鱼（300只鱼，初始重量为2.4±0.12 g）随机分配到12个水箱中，每千克饲料分别饲喂0（对照），5、10和15 g CBE，持续8周。根据结果​​，饲喂15 g / kg CBE的鱼的皮肤粘液样本中的溶菌酶活性，碱性磷酸酶和总免疫球蛋白（Ig）显着提高（P <0.05）。此外，与对照组相比，膳食CBE对血清样品的溶菌酶活性，补体成分和IgM含量有积极影响。而且，随着饮食中CBE的增加，单核细胞和淋巴细胞的数量也显着增加（P  <0.05）。在15 g / kg CBE处理中观察到最高的肿瘤坏死因子（TNF-α，TNF-2α）和溶菌酶的mRNA水平。攻击试验后，在饲喂15 g / kg CBE的鱼中观察到最高相对存活百分比值（60％）。我们得出的结论是，饮食中的CBE，尤其是15 g / kg的CBE，通过刺激先天免疫力和某些炎症性细胞因子以及抗病性，对金鱼具有免疫调节作用。A.亲水性。