MicroRNAs (miRNAs) are non-coding RNAs that are associated with adipokine homeostasis and insulin resistance. Whereas, smoking can disturb metabolic homeostasis. Present study was aimed to investigate the level of miRNA-141 in experimental animal model that were exposed with graded doses of nicotine. We further aimed to investigate the possible interplay of miRNA-141 expression change with adipokine homeostasis and occurrence of insulin resistance in nicotine-exposed experimental animals. Nicotine (0.5, 1.0, 3.0 and 6.0 mg/Kg) was administered to early adolescent; postnatal days ranging from 25 to 30 Wistar rats for one month. Serum was analyzed for leptin, adipokines, IL-6, MDA, HbA1c, insulin, G6PDH, hexokinase, and lipid profile. While miRNA-141 expression level was determined in plasma. Higher doses of nicotine were associated with higher glucose, HbA1c, leptin, IL-6, MDA and lipids levels, while, insulin, adiponectin, G6PDH, hexokinase and HDL levels were lower. Higher doses of nicotine also impaired glucose tolerance and exhibited significant increase in miR-141 expression signifying that nicotine exposure may influence adipokines regulation altering glycemic profile. This is accompanied with aggravated inflammatory responses where genetic expression of miRNA-141 can be an accessible biomarker for metabolic disturbances with insulin resistance and glucose intolerance.

MicroRNA（miRNA）是与脂肪因子体内稳态和胰岛素抵抗相关的非编码RNA。而吸烟会干扰代谢稳态。本研究旨在研究暴露于分级剂量烟碱的实验动物模型中miRNA-141的水平。我们进一步的目的是调查暴露于尼古丁的实验动物中miRNA-141表达变化与脂肪因子稳态和胰岛素抵抗发生的可能相互作用。尼古丁（0.5、1.0、3.0和6.0 mg / Kg）用于青春期早期；25至30只Wistar大鼠的产后天数为一个月。分析血清中的瘦素，脂肪因子，IL-6，MDA，HbA1c，胰岛素，G6PDH，己糖激酶和脂质分布。同时测定血浆中的miRNA-141表达水平。较高剂量的烟碱与较高的葡萄糖，HbA1c，瘦素，IL-6，MDA和脂质水平相关，而胰岛素，脂联素，G6PDH，己糖激酶和HDL水平较低。更高剂量的尼古丁也会损害葡萄糖耐量，并且在miR-141表达上显着增加，这表明尼古丁暴露可能会影响改变血糖谱的脂肪因子调节。这伴随着严重的炎症反应，其中miRNA-141的基因表达可能是胰岛素抵抗和葡萄糖耐受不良的代谢紊乱的可及生物标记。更高剂量的尼古丁也会损害葡萄糖耐量，并且在miR-141表达上显着增加，这表明尼古丁暴露可能会影响改变血糖谱的脂肪因子调节。这伴随着严重的炎症反应，其中miRNA-141的基因表达可能是胰岛素抵抗和葡萄糖耐受不良的代谢紊乱的可及生物标记。更高剂量的尼古丁也会损害葡萄糖耐量，并且在miR-141表达上显着增加，这表明尼古丁暴露可能会影响改变血糖谱的脂肪因子调节。这伴随着严重的炎症反应，其中miRNA-141的基因表达可能是胰岛素抵抗和葡萄糖耐受不良的代谢紊乱的可及生物标记。