Abstract Continuous crystallization of proteins at short residence time and high supersaturation level is attractive in terms of the space-time yield and production efficiency. Nevertheless, it is rarely pursued due to its less-than-desirable crystal size distribution (CSD) characterized by the abundance of small crystals due to high nucleation rate. The small crystals were prone to random agglomeration, resulting in poor crystals’ residence time distribution, hence low CSD’s reproducibility. Herein we developed a segmented slug flow crystallizer (SFC) design operated at short residence time (

中文翻译：

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提高在短停留时间运行的连续段塞流结晶器中产生的蛋白质晶体粒度分布的再现性

摘要 在短停留时间和高过饱和度下连续结晶蛋白质在时空产率和生产效率方面具有吸引力。然而，由于其不太理想的晶体尺寸分布 (CSD)，其特点是由于高成核率导致大量小晶体，因此很少有人追求它。小晶体容易随机团聚，导致晶体的停留时间分布差，因此 CSD 的重现性低。在此，我们开发了一种分段式段塞流结晶器 (SFC) 设计，其运行时间很短（