Abstract This study examined the nutritional value of solvent-extracted canola meal (CM) as a replacement for fish meal (FM) in practical diets for spotted rose snapper (Lutjanus guttatus). Four isonitrogenous (490 g/kg crude protein), isoenergetic (19.03 kJ/g) diets containing 0 g/kg (CM0), 150 g/kg (CM150), 300 g/kg (CM300), or 450 g/kg (CM450) of CM protein instead of FM protein were used. All CM diets were supplemented with phytase and protease enzymes; DL-methionine was supplemented to the CM450 diet. Each diet was fed to triplicate groups of 15 fish (average body weight 36.2 ± 0.03 g) per tank to apparent satiation three times a day for 70 days. A one-way ANOVA showed that the specific growth rate (SGR) was significantly lower when CM inclusion was 26.67% or higher, replacing 450 g/kg of fish meal protein or more. There was a significant negative relationship between growth response and the level of FM protein replacement. Survival and dry feed intake (DFI) were unaffected by fish meal replacement. The protein efficiency ratio (PER) was highest at the lowest CM inclusion level. Hematological parameters, including hematocrit, protein, hemoglobin, triglycerides, and cholesterol, did not vary between treatments. Juvenile fish fed the 450 g/kg CM diet showed the lowest protein content. The apparent digestibility coefficient (ADC) of dry matter and energy were significantly reduced by CM addition. Our results show that CM can be a suitable alternative protein source for spotted rose snapper juveniles heavier than 37 g. Under the experimental conditions tested in this study, up to 300 g/kg FM protein can be replaced by CM protein with no significant adverse effects on growth, feed utilization, hematological parameters, or body composition in this species.

中文翻译：

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双低菜粕作为幼鱼粉替代鱼粉的营养评估：对生长性能、血液学参数、身体成分和营养消化率的影响

摘要 本研究检验了溶剂提取的双低菜粕 (CM) 作为鱼粉 (FM) 替代品在斑点玫瑰鲷 (Lutjanus guttatus) 的实际日粮中的营养价值。四种含 0 g/kg (CM0)、150 g/kg (CM150)、300 g/kg (CM300) 或 450 g/kg (使用 CM 蛋白的 CM450) 代替 FM 蛋白。所有 CM 日粮都添加了植酸酶和蛋白酶；DL-甲硫氨酸被补充到CM450饮食中。将每种饮食喂给每罐 15 条鱼（平均体重 36.2 ± 0.03 克）的三份重复组，每天 3 次，持续 70 天。单向方差分析表明，当 CM 含量为 26.67% 或更高，替代 450 g/kg 或更多鱼粉蛋白时，比增长率 (SGR) 显着降低。生长反应与 FM 蛋白替代水平之间存在显着的负相关关系。鱼粉替代品不会影响存活率和干饲料摄入量 (DFI)。蛋白质效率比 (PER) 在最低 CM 包含水平时最高。血液学参数，包括血细胞比容、蛋白质、血红蛋白、甘油三酯和胆固醇，在治疗之间没有变化。饲喂 450 g/kg CM 饮食的幼鱼表现出最低的蛋白质含量。添加CM显着降低了干物质和能量的表观消化率系数（ADC）。我们的结果表明，CM 可以成为体重超过 37 克的斑点玫瑰鲷鱼幼鱼的合适替代蛋白质来源。在本研究测试的实验条件下，