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Replacing corn grain with corn gluten feed: Effects on the rumen microbial protein synthesis, functional bacterial groups and epithelial amino acid chemosensing in growing goats
Animal Feed Science and Technology ( IF 2.5 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.anifeedsci.2020.114684
Jian Wu , Xiaoli Zhang , Rong Wang , Min Wang , Zhixiong He , Zhiliang Tan , Jinzhen Jiao

Abstract Corn gluten feed is rich in crude protein and has been widely used in ruminant diets as an economical substitution for corn grain. This study was conducted to assess the effect of corn grain replaced with corn gluten feed on the ruminal microbial protein (MCP) synthesis, amino acid profile, and mucosal amino acid chemosensing pathway in growing goats, as well as the related mechanisms involved in the ruminal nitrogen metabolism. Twenty male healthy goats with similar body weight (14.5 ± 2.2 kg) and age (1.2 ± 0.2 yr) were randomly assigned to either a corn gluten diet (CGF, 500 g/kg DM) or a corn grain diet (CRN, 500 g/kg DM) (n = 10/diet). The results showed that compared with the CRN diet, goats fed with CGF diet had greater (P 0.05) were noted for the copy numbers of other well-known amylolytic and cellulolytic bacteria, and free amino acids (FAAs) in the rumen fluids between the two diets. In respect to mucosal amino acid chemosensing pathway, no significant differences (P > 0.05) were observed in mucosal FAAs profile, mRNA expression of anionic, neutral and cationic amino acid transporters, as well as concentration of second messengers in the ruminal mucosa in goats fed the diets. In summary, in response to CGF substitution, dietary CP was preferred to be accumulated in ammonia, and lower ruminal bacteria number decreased ruminal MCP synthesis. Further, CGF substitution did not suppress mucosal amino acid chemosensing system, along with elevated ruminal pH, indicating that the CGF is a better alternative when formulating diets for growing goats.

中文翻译:

用玉米蛋白饲料代替玉米粒:对生长山羊瘤胃微生物蛋白质合成、功能菌群和上皮氨基酸化学传感的影响

摘要 玉米蛋白饲料含有丰富的粗蛋白,作为玉米粒的经济替代品被广泛应用于反刍动物饲料中。本研究旨在评估用玉米麸质饲料代替玉米粒对生长山羊瘤胃微生物蛋白 (MCP) 合成、氨基酸谱和粘膜氨基酸化学传感途径的影响,以及瘤胃中涉及的相关机制。氮代谢。20 只体重 (14.5 ± 2.2 kg) 和年龄 (1.2 ± 0.2 岁) 相似的雄性健康山羊被随机分配到玉米麸质饮食 (CGF, 500 g/kg DM) 或玉米谷物饮食 (CRN, 500 g /kg DM)(n = 10/饮食)。结果表明,与 CRN 日粮相比,饲喂 CGF 日粮的山羊的其他知名淀粉分解菌和纤维素分解菌的拷贝数更高(P = 0.05),和两种日粮之间瘤胃液中的游离氨基酸 (FAA)。在粘膜氨基酸化学传感途径方面,饲喂山羊的粘膜FAA谱、阴离子、中性和阳离子氨基酸转运蛋白的mRNA表达,以及瘤胃粘膜中第二信使的浓度均无显着差异(P > 0.05)饮食。总之,为了响应 CGF 替代,日粮 CP 更倾向于在氨中积累,较低的瘤胃细菌数量减少了瘤胃 MCP 的合成。此外,CGF 取代不抑制粘膜氨基酸化学感应系统,以及升高的瘤胃 pH 值,表明 CGF 是配制生长山羊日粮时更好的替代品。在饲喂日粮的山羊的粘膜 FAA 谱、阴离子、中性和阳离子氨基酸转运蛋白的 mRNA 表达以及瘤胃粘膜中第二信使的浓度方面没有观察到显着差异(P > 0.05)。总之,为了响应 CGF 替代,日粮 CP 更倾向于在氨中积累,较低的瘤胃细菌数量减少了瘤胃 MCP 的合成。此外,CGF 取代不抑制粘膜氨基酸化学感应系统,以及升高的瘤胃 pH 值,表明 CGF 是配制生长山羊日粮时更好的替代品。在饲喂日粮的山羊的粘膜 FAA 谱、阴离子、中性和阳离子氨基酸转运蛋白的 mRNA 表达以及瘤胃粘膜中第二信使的浓度方面没有观察到显着差异(P > 0.05)。总之,为了响应 CGF 替代,日粮 CP 更倾向于在氨中积累,较低的瘤胃细菌数量减少了瘤胃 MCP 的合成。此外,CGF 取代不抑制粘膜氨基酸化学感应系统,以及升高的瘤胃 pH 值,表明 CGF 是配制生长山羊日粮时更好的替代品。总之,为了响应 CGF 替代,日粮 CP 更倾向于在氨中积累,较低的瘤胃细菌数量减少了瘤胃 MCP 的合成。此外,CGF 取代不抑制粘膜氨基酸化学感应系统,以及升高的瘤胃 pH 值,表明 CGF 是配制生长山羊日粮时更好的替代品。总之,为了响应 CGF 替代,日粮 CP 更倾向于在氨中积累,较低的瘤胃细菌数量减少了瘤胃 MCP 的合成。此外,CGF 取代不抑制粘膜氨基酸化学感应系统,以及升高的瘤胃 pH 值,表明 CGF 是配制生长山羊日粮时更好的替代品。
更新日期:2020-12-01
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