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Microbial Distribution and Diversity of Soil Around a Manganese Mine Area
Water, Air, & Soil Pollution ( IF 3.8 ) Pub Date : 2020-09-28 , DOI: 10.1007/s11270-020-04878-3
Yuwei Xiang , Yiqie Dong , Suyun Zhao , Fan Ye , Yi Wang , Min Zhou , Haobo Hou

From 12 mining areas surrounding a manganese mining area in Guangxi, soil samples were collected at different distances from the center of the mining area and different sampling points at the same distance. 16S rRNA gene sequencing was used in extracting and amplifying DNA in the soil samples, and double-terminal gene sequence library comparison was performed. Through OUT clustering, species community, species diversity, and inter-group difference analyses, the composition of microbial community and biological diversity in the mining soil was evaluated. Results showed that the soil around the mining area was affected by heavy metals, and species complexity was positively correlated with distance. Proteobacteria, Firmicutes, Bacteroidetes, and Acidobacteria were the dominant species with high abundance in some soil samples because of their pollution tolerance. The samples closer to the center were more disturbed by mining activities, and species diversity sequencing based on observed species index and Shannon index was consistent with that of cluster analysis. Differences among the sample groups at 2 km from the center were the smallest, and species diversity had the highest similarity. Differences among the sample groups at 1 km from the center were the largest, and species composition significantly varied. Microorganism distribution is an important indicator of soil ecological characteristics, and the analysis of species and diversity of soil microorganisms is important to the assessment of the impact of mining activities and the extent of soil contamination.



中文翻译:

锰矿区周围土壤微生物分布与多样性

从广西锰矿区周围的12个矿区中,以距矿区中心不同距离的距离采集了土壤样品,并以相同距离采集了不同的采样点。用16S rRNA基因测序法提取和扩增土壤样品中的DNA,并进行双末端基因序列文库比较。通过OUT聚类,物种群落,物种多样性和群体间差异分析,对采矿土壤中的微生物群落组成和生物多样性进行了评估。结果表明,矿区周围土壤受到重金属的影响,物种复杂度与距离呈正相关。变形杆菌,硬菌,拟杆菌,酸性细菌和酸性细菌由于其耐污染性,在某些土壤样品中是高丰度的优势种。靠近中心的样本受采矿活动的干扰更大,基于观察到的物种指数和香农指数的物种多样性排序与聚类分析的结果一致。距中心2 km的样本组之间的差异最小,而物种多样性的相似性最高。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。靠近中心的样本受到采矿活动的干扰更大,基于观察到的物种指数和香农指数的物种多样性排序与聚类分析的结果一致。距中心2 km的样本组之间的差异最小,而物种多样性的相似性最高。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。靠近中心的样本受采矿活动的干扰更大,基于观察到的物种指数和香农指数的物种多样性排序与聚类分析的结果一致。距中心2 km的样本组之间的差异最小,而物种多样性的相似性最高。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。基于观察物种指数和香农指数的物种多样性排序与聚类分析相吻合。距中心2 km的样本组之间的差异最小,而物种多样性的相似性最高。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。基于观测物种指数和香农指数的物种多样性排序与聚类分析相吻合。距中心2 km的样本组之间的差异最小,而物种多样性的相似性最高。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。距中心1公里处的样本组之间的差异最大,并且物种组成显着变化。微生物的分布是土壤生态特征的重要指标,对土壤微生物的种类和多样性进行分析对于评估采矿活动的影响和土壤污染程度具有重要意义。

更新日期:2020-09-28
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