Human milk proteins are known as vital molecules for infant development and growth. Tissue factor is one of these human milk proteins that its role in human milk has not been cleared yet. Therefore, the first aim of this study was to detect the tissue factor activity of human milk and also was to investigate the effect of extended freezer storage on the milk tissue factor activity. The relationship between the tissue factor activity and macronutrient content and pH of milk was also investigated in this study. Under this aim, mature human milk samples were obtained from 8 healthy women. Collected human milk samples were pooled and divided into aliquots that were stored at − 20 °C until the day to be analyzed. Milk tissue factor activity, protein, fat, lactose, energy, water, density, and pH levels were determined for up to six months. By two months from the freezing, tissue factor activity did not significantly change but significantly decreased at the end of the six months. From the first month to six months from freezing, lactose, protein, fat, and energy levels showed a significant decline. Milk pH did not change with freezing at the end of 6 months. In conclusion, TF activity maintained its first-day activity until the second month after being pumped. The increased interest in breast milk leads us to believe that the gap existing in the knowledge of breast milk bioactive components like TF will be complemented with new research data.

人乳蛋白被称为婴儿发育和成长的重要分子。组织因子是这些人乳蛋白中的一种，尚未明确其在人乳中的作用。因此，这项研究的第一个目标是检测人乳的组织因子活性，并且还研究延长冷冻储存时间对乳组织因子活性的影响。在这项研究中，还研究了组织因子活性与牛奶中大量营养素含量和pH之间的关系。在此目标下，从8位健康女性中获得了成熟的人乳样品。收集收集的人乳样品并分成等分试样，将其等份保存在-20°C直至进行分析的当天。确定长达六个月的牛奶组织因子活性，蛋白质，脂肪，乳糖，能量，水，密度和pH值。从冻结开始到两个月，组织因子活性没有显着变化，但在六个月末显着下降。从冻结的第一个月到六个月，乳糖，蛋白质，脂肪和能量水平显着下降。牛奶的pH值在6个月末没有随冻结而变化。总之，TF的活动一直保持其第一天的活动，直到抽水后的第二个月为止。对母乳的兴趣增加使我们相信，母乳生物活性成分（如TF）的知识方面的差距将得到新的研究数据的补充。能量水平显着下降。牛奶的pH值在6个月末没有随冻结而变化。总之，TF的活动一直保持其第一天的活动，直到抽水后的第二个月为止。对母乳的兴趣增加使我们相信，母乳生物活性成分（如TF）的知识方面的差距将得到新的研究数据的补充。能量水平显着下降。牛奶的pH值在6个月末没有随冻结而变化。总之，TF的活动一直保持其第一天的活动，直到抽水后的第二个月为止。对母乳的兴趣增加使我们相信，母乳生物活性成分（如TF）的知识方面的差距将得到新的研究数据的补充。