Iris is a perennial flowering plant, usually cultivated through seeds or bulbs. However, due to the limitations of traditional reproduction, the establishment of a callus regeneration system is particularly important, and callus induction and plant regeneration are the keys to transgenic technology. The callus regeneration system was studied using the I. sanguinea flower stem as explants. The experiment investigated the impact of different disinfectant solution concentrations, disinfection exposure times, and hormone concentrations on the explant growth status at various culture stages. The research analyzed the impact of the experimental variables on explant regeneration to determine the optimum callus regeneration system for I. sanguinea. In this research, it was determined that when I. sanguinea flower stems, that were used for explants, were treated with 75% alcohol for 30 s and 2% NaClO for 8 min for disinfection, there was a 54.48% callus induction rate. The callus induction medium was Murashige and Skoog medium (MS) + 6-benzylaminopurine (6-BA) 1.0 mg/L + 1-naphthylacetic acid (NAA) 0.2 mg/L + 2,4-dichlorophenoxyacetic acid (2,4-D) 2.0 mg/L for 40 d. The optimum medium for callus multiplication was MS + 6-BA 0.5 mg/L + 2,4-D 0.5 mg/L, while the optimum medium for callus adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + kinetin (KT) 0.5 mg/L, 60 d. The adventitious bud differentiation rate from flower stem calli was 46.03%.

鸢尾是多年生开花植物，通常通过种子或鳞茎栽培。然而，由于传统繁殖的局限性，愈伤组织再生系统的建立尤为重要，愈伤组织的诱导和植物再生是转基因技术的关键。研究了愈伤组织再生系统，该方法以番红花I. sanguenea花茎为外植体。该实验研究了不同消毒液浓度，消毒暴露时间和激素浓度对不同培养阶段外植体生长状态的影响。该研究分析了实验变量对外植体再生的影响，以确定最佳的愈伤组织I. sanguinea的愈伤组织再生系统。。在这项研究中，确定了将用于外植体的I. sanguinea花茎用75％酒精处理30 s，用2％NaClO处理8 min进行消毒时，有54.48％的愈伤组织诱导率。愈伤组织诱导培养基为Murashige and Skoog培养基（MS）+ 6-苄基氨基嘌呤（6-BA）1.0 mg / L + 1-萘乙酸（NAA）0.2 mg / L + 2,4-二氯苯氧基乙酸（2,4-D ）2.0毫克/升40 d。愈伤组织繁殖的最佳培养基为MS + 6-BA 0.5 mg / L + 2,4-D 0.5 mg / L，而愈伤组织不定芽诱导的最佳培养基为MS + 6-BA 1.0 mg / L + NAA 0.2 mg / L +激动素（KT）0.5 mg / L，60天。花茎愈伤组织不定芽分化率为46.03％。