Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose fraction was obtained for reaction with the whole-cell biocatalyst. FESEM and FTIR analyses were used to observe morphological and compositional changes of pineapple pomace respectively after pretreatment. Factors affecting hydrolysis reaction were investigated and optimized using the Box-Behnken Design. The highest amount of reducing sugar was produced at pH 7.5, cell loading of 100 g/L wet cell weight, and temperature of 30 °C. The amount of reducing sugar produced was 2.129 mg/ml. HPLC analysis indicated that the XOS produced were xylobiose and xylotriose with a total yield of 5.4 mg/g of pineapple hemicellulose. FESEM analysis on the surface structure of pineapple pomace after the hydrolysis reaction showed clear signs of degradation by xylanase. Based on the results presented, it can be deduced that the application of cell surface display on E. coli for degradation of lignocellulosic biomass is possible and should be explored as it offers great potential for the production of XOS in industry.

木聚糖酶在大肠杆菌上的细胞表面展示用于从菠萝渣中水解半纤维素。研究了木质纤维素生物质生物转化为木寡糖（XOS）的可行性。在本研究中，对菠萝渣进行了预处理，并获得了半纤维素馏分与全细胞生物催化剂反应。用FESEM和FTIR分析分别观察预处理后菠萝果渣的形态和组成变化。使用Box-Behnken设计调查和优化了影响水解反应的因素。在pH 7.5，细胞负荷为100 g / L湿细胞重量，温度为30°C时，产生的还原糖量最高。产生的还原糖的量为2.129mg / ml。HPLC分析表明，产生的XOS为木糖和木三糖，总产率为5。4 mg / g菠萝半纤维素。水解反应后菠萝果渣的表面结构的FESEM分析显示出明显的木聚糖酶降解迹象。根据给出的结果，可以推断出细胞表面显示在降解木质纤维素生物质的大肠杆菌是可能的，应加以探索，因为它为工业生产XOS提供了巨大的潜力。